Gyongyossy-Issa M I, Khachatourians G G
Tissue Cell. 1985;17(6):801-9. doi: 10.1016/0040-8166(85)90037-0.
We describe a new type of cell fragmentation in P815Y mastocytoma cells yielding one, large, enucleated 'minicell' at a time per parent tumour cell. These tumour minicells (TMC) arise spontaneously in semi-synthetic medium during early stationary phase of the growth curve. Their diameter comprises 21-30% of that of the parent cell line. Separated from parent tumour cells, they are non-tumorigenic. TMC can induce cytotoxic T cell activity against the parent tumour cell line greater cytotoxicity being observed against the P815Y line than an H-2-identical line, L1210. TMC may provide a new tool adaptable to the study of host-tumour relationships, cell size regulation or the mechanistic aspects of cell membrane function.
我们描述了一种在P815Y肥大细胞瘤细胞中出现的新型细胞分裂方式，每个亲代肿瘤细胞每次产生一个大的、无核的“微细胞”。这些肿瘤微细胞（TMC）在生长曲线的早期静止期于半合成培养基中自发产生。它们的直径为亲代细胞系直径的21% - 30%。与亲代肿瘤细胞分离后，它们不具有致瘤性。TMC可诱导细胞毒性T细胞针对亲代肿瘤细胞系产生活性，相较于H - 2相同的L1210细胞系，对P815Y细胞系观察到更强的细胞毒性。TMC可能为研究宿主 - 肿瘤关系、细胞大小调节或细胞膜功能的机制方面提供一种新工具。
