针对牛IgA、IgM、IgG1和IgG2的重链特异性抗血清的制备。

Preparation of heavy chain specific antisera to bovine IgA, IgM, IgG1 and IgG2.

作者信息

Nielsen K, Duncan J R, Stemshorn B

出版信息

Vet Immunol Immunopathol. 1985 Aug;9(4):361-9. doi: 10.1016/0165-2427(85)90065-0.

Abstract

Goats, guinea pigs and rabbits were immunized with bovine IgM or with intact molecules, heavy chains, Fc portions or light chains of bovine IgG1 and IgG2. Rabbits and guinea pigs were immunized with bovine secretory IgA. Goats and guinea pigs produced heavy chain specific antisera to intact IgM whereas rabbits produced anti-light chain antibody and in one instance anti-alpha 2-macroglobulin antibody in addition to the anti-mu response. Goats and guinea pigs produced antisera to bovine IgG1 and IgG2 and their Fc portions that needed little absorption to render them monospecific for the heavy chain. In addition to antibody to the heavy chains, rabbits produced anti-light chain antibody when immunized with intact IgG1 or IgG2 molecules. These latter sera, and those produced by rabbits immunized with Fc portions of IgG1 or IgG2 required extensive absorption before they were monospecific for their respective heavy chains. Heavy chains were poor immunogens in all three species. Rabbits immunized with IgA produced both anti-alpha and anti-light chain antibodies while guinea pigs produced sera with antibody activity to the alpha chain only.

摘要

用牛 IgM 或牛 IgG1 和 IgG2 的完整分子、重链、Fc 片段或轻链对山羊、豚鼠和兔子进行免疫。用牛分泌型 IgA 对兔子和豚鼠进行免疫。山羊和豚鼠产生了针对完整 IgM 的重链特异性抗血清，而兔子除了产生抗 μ 反应外，还产生了抗轻链抗体，在一个实例中还产生了抗 α2-巨球蛋白抗体。山羊和豚鼠产生了针对牛 IgG1 和 IgG2 及其 Fc 片段的抗血清，这些抗血清只需很少的吸收就能使其对重链具有单特异性。除了针对重链的抗体外，兔子在用完整的 IgG1 或 IgG2 分子免疫时还产生了抗轻链抗体。这些后一种血清，以及用 IgG1 或 IgG2 的 Fc 片段免疫兔子产生的血清，在它们对各自的重链具有单特异性之前需要大量吸收。重链在所有这三个物种中都是弱免疫原。用 IgA 免疫兔子产生了抗 α 链和抗轻链抗体，而豚鼠产生的血清仅具有针对 α 链的抗体活性。