Nielsen K, Duncan J R
Res Vet Sci. 1981 Sep;31(2):147-50.
A technique for producing specific antibovine IgG2 antibodies is described. The method relies on the abrogation of the class-specific antibody response of guinea pigs to bovine IgG1 by intravenous injection of goat serum immediately before immunisation in the foot pads with bovine IgG2 in adjuvant. Of the 10 resulting antisera, six were judged monospecific for IgG2 by immunoelectrophoresis but, of these, two antisera gave a very faint line in gel diffusion using IgG1 as the antigen. Radial immunodiffusion studies indicated that the strength of the antisera, using IgG2 as the antigen, was similar to antisera of guinea pigs not injected with goat serum before absorption with bovine IgG1. For guinea pigs injected with goat serum, using bovine IgG1 as an immunogen did not result in the production of subclass specific antisera, rather, the specificities were similar to those of animals not receiving goat serum. This data is compared to absorption studies of goat antibovine IgG1 and IgG2 antisera. The relationships of goat and bovine IgG subclasses are discussed.
本文描述了一种制备特异性抗牛IgG2抗体的技术。该方法依赖于在豚鼠足垫用佐剂中的牛IgG2免疫前，立即静脉注射山羊血清，从而消除豚鼠对牛IgG1的类特异性抗体反应。在产生的10份抗血清中，通过免疫电泳判断有6份对IgG2具有单特异性，但其中有2份抗血清在以IgG1为抗原的凝胶扩散试验中出现非常微弱的条带。放射免疫扩散研究表明，以IgG2为抗原时，这些抗血清的强度与在用牛IgG1吸收前未注射山羊血清的豚鼠抗血清相似。对于注射了山羊血清的豚鼠，以牛IgG1作为免疫原不会产生亚类特异性抗血清，相反，其特异性与未接受山羊血清的动物相似。将该数据与山羊抗牛IgG1和IgG2抗血清的吸收研究进行了比较。并讨论了山羊和牛IgG亚类之间的关系。
