Section of Infectious Diseases, Department of Internal Medicine, School of Medicine, Yale University, New Haven, Connecticut, USA.
mBio. 2024 Nov 13;15(11):e0243324. doi: 10.1128/mbio.02433-24. Epub 2024 Oct 7.
The mosquito plays a critical role in the transmission of viral diseases, including Zika virus, which poses significant public health challenges. Understanding the complex interactions between mosquitoes and viruses is paramount for the development of effective control strategies. In this study, we demonstrate that silencing the adiponectin receptor-like protein (AaARLP) results in a reduction of Zika virus infection. Transcriptomic analysis identified alterations in several trypsin genes and further revealed that -knockdown mosquitoes had diminished trypsin activity. Moreover, silencing of selected resulted in a similar delay in Zika virus infection in mosquitoes, further highlighting the connection between the AaARLP and trypsin. Overall, our findings demonstrate that AaARLP signaling is important for Zika virus infection of .
Arboviruses pose a significant threat to public health, with mosquitoes, especially , being a major vector for their transmission. Gaining insight into the complex interaction between mosquitoes and viruses is essential to build successful control strategies. In this study, we identified a novel pathway connecting the adiponectin receptor-like protein and its association with trypsin, key enzymes involved in blood digestion. Furthermore, we demonstrated the significance of signaling via the adiponectin receptor-like protein in virus infection within the mosquito. Together, our discoveries illuminate mosquito metabolic pathways essential in viral infection.
蚊子在病毒疾病的传播中起着关键作用，包括寨卡病毒，这对公共卫生构成了重大挑战。了解蚊子和病毒之间的复杂相互作用对于制定有效的控制策略至关重要。在这项研究中，我们证明了沉默脂联素受体样蛋白（AaARLP）会导致寨卡病毒感染减少。转录组分析鉴定出几种胰蛋白酶基因的改变，并进一步表明 - 敲低蚊子的胰蛋白酶活性降低。此外，沉默选定的基因也会导致蚊子中寨卡病毒感染的类似延迟，这进一步强调了 AaARLP 与胰蛋白酶之间的联系。总的来说，我们的研究结果表明，AaARLP 信号对于寨卡病毒感染蚊子至关重要。
虫媒病毒对公共卫生构成重大威胁，蚊子尤其是主要传播媒介。深入了解蚊子和病毒之间的复杂相互作用对于建立成功的控制策略至关重要。在这项研究中，我们确定了一种连接脂联素受体样蛋白及其与参与血液消化的关键酶胰蛋白酶之间的新途径。此外，我们证明了脂联素受体样蛋白信号在蚊子中病毒感染的重要性。总之，我们的发现阐明了蚊子代谢途径在病毒感染中的重要性。
