Giorgio Marianela, Ramírez Ladino Kelly Alejandra, López Guido, Sosa Rojas Maricel, Outon Estela, Delfino Cecilia María
Laboratorio de Virología, Hospital Interzonal General de Agudos 'Dr. Pedro Fiorito', Avellaneda, Buenos Aires, Argentina.
Universidad de Buenos Aires (UBA) - Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPAM). Ciudad Autónoma de Buenos Aires, Argentina.
Eur J Gastroenterol Hepatol. 2025 Jan 1;37(1):70-76. doi: 10.1097/MEG.0000000000002857. Epub 2024 Oct 8.
Hepatitis D virus (HDV) RNA-positive cases with total anti-HDV antibodies nonreactive were documented. Moreover, HDV infection was observed in subjects with occult hepatitis B virus infection. The prevalence of HDV infection in Argentina is low; however, further research in different populations is needed.
This study aimed to perform synchronous HDV detection in reactive hepatitis B virus patients treated in a public hospital in the province of Buenos Aires, Argentina, some of whom were coinfected with hepatitis C virus and/or HIV. A total of 189 hepatitis B virus-reactive serum samples with or without hepatitis C virus and/or HIV coinfection were synchronously analyzed for anti-HDV antibodies and HDV RNA.
HDV prevalence was 4.2% with HDV RNA found in 61 samples, most of which were nonreactive to anti-HDV antibodies and hepatitis B surface antigen. Genotype 1 was identified in all HDV sequences. Moreover, triple and quadruple infections were observed, showing a high frequency of HDV infection in hospitalized patients not following the recommended diagnostic algorithm.
This study is evidence that the synchronous testing of anti-HDV antibodies and HDV RNA is necessary for the diagnosis of HDV infection in Argentina. Finally, further research is necessary to identify high-risk populations and improve prevention and control strategies for triple and quadruple infections and their potential consequences.
已记录到丁型肝炎病毒（HDV）RNA阳性且抗-HDV总抗体无反应的病例。此外，在隐匿性乙型肝炎病毒感染的受试者中也观察到HDV感染。阿根廷HDV感染的患病率较低；然而，需要对不同人群进行进一步研究。
本研究旨在对在阿根廷布宜诺斯艾利斯省一家公立医院接受治疗的反应性乙型肝炎病毒患者进行同步HDV检测，其中一些患者合并感染丙型肝炎病毒和/或人类免疫缺陷病毒。对总共189份有或无丙型肝炎病毒和/或人类免疫缺陷病毒合并感染的乙型肝炎病毒反应性血清样本同步分析抗-HDV抗体和HDV RNA。
HDV患病率为4.2%，在61份样本中检测到HDV RNA，其中大多数对抗-HDV抗体和乙型肝炎表面抗原无反应。在所有HDV序列中均鉴定出1型基因型。此外，观察到三重和四重感染，表明在未遵循推荐诊断算法的住院患者中HDV感染频率较高。
本研究证明，在阿根廷，抗-HDV抗体和HDV RNA的同步检测对于HDV感染的诊断是必要的。最后，有必要进一步研究以确定高危人群，并改进针对三重和四重感染及其潜在后果的预防和控制策略。
