Department of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, Pakistan.
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan.
Int J Biol Macromol. 2024 Nov;281(Pt 3):135896. doi: 10.1016/j.ijbiomac.2024.135896. Epub 2024 Oct 5.
Hydrogel wound dressings provide a moist environment, which promotes the formation of granulation tissue and epithelium in the wound area, accelerating the wound healing process. There have been numerous approaches to skin wound management and treatment, but the limitations of current methods highlight the need for more effective alternatives. A Chitosan polyherbal hydrogel integrated with AgNPs was synthesized to assess its wound-healing potential both in vitro and in vivo. The AgNPs were synthesized using Calotropis procera leaf extract and characterized via X-ray diffraction analysis (XRD), Scanning electron microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FT-IR). In swelling kinetic analysis, the hydrogel's weight reached its maximum at 8 h of incubation and began to decrease from 12 h up to 72 h (49 % ± 6.04). The hydrogel formulation demonstrated strong antimicrobial potential against E. coli and S. aureus with an inhibition zone of 18 mm and 25 mm, respectively. Furthermore, in mice studies, the formulation exhibited significant wound size reduction within 12 days, supported by histopathology analysis revealing higher angiogenic potential compared to commercial hydrogels. The concentrations of IL-6 and TNF-α in CS-polyherbal/AgNPs hydrogel were 500 pg/ml and 125 pg/ml, respectively. Additionally, a network pharmacology approach identified 11 chemical constituents in Aloe vera, Azadirachta indica, and Alternanthera brasiliana extracts, along with 326 potential targets, suggesting the superior wound healing properties of this formulation compared to commercially available hydrogels.
水凝胶伤口敷料提供了一个湿润的环境，促进了伤口区域肉芽组织和上皮的形成，加速了伤口愈合过程。人们已经提出了许多方法来管理和治疗皮肤伤口，但目前方法的局限性突出表明需要更有效的替代方法。本研究合成了一种壳聚糖草药水凝胶，与 AgNPs 结合，以评估其在体外和体内的伤口愈合潜力。AgNPs 是使用 Calotropis procera 叶提取物合成的，并通过 X 射线衍射分析（XRD）、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）进行了表征。在溶胀动力学分析中，水凝胶的重量在孵育 8 小时时达到最大值，并从 12 小时开始减少到 72 小时（49%±6.04）。该水凝胶配方对大肠杆菌和金黄色葡萄球菌具有很强的抗菌潜力，抑菌圈分别为 18mm 和 25mm。此外，在小鼠研究中，该配方在 12 天内显著减少了伤口面积，组织病理学分析显示其具有比商业水凝胶更高的血管生成潜力。CS-草药/AgNPs 水凝胶中的 IL-6 和 TNF-α 浓度分别为 500pg/ml 和 125pg/ml。此外，网络药理学方法鉴定出库拉索芦荟、印楝和巴西野牡丹提取物中的 11 种化学成分，以及 326 种潜在靶点，表明该配方的伤口愈合性能优于市售水凝胶。
