Yu Yun-Sang, Kim Jin-Se, Jiménez Brenda Irene Medina, Kim Tae-Wuk, Cho Sung-Jin
School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea.
Department of Life Sciences, Hanyang University, Seoul, 133-791, Republic of Korea.
Genes Genomics. 2018 Jul;40(7):747-753. doi: 10.1007/s13258-018-0683-3. Epub 2018 Apr 4.
The earthworm Perionyx excavatus is a species highly capable of bidirectional regeneration. Pair-rule genes are thought to have an ancestral function in arthropod segmentation. However, orthologs in annelids (i.e. Capitella teleta) do not exhibit segmental expression in the ectoderm or mesoderm. Their role in regeneration is currently unclear. Here, we report the expression profile of primary pair-rule genes (Pex-EvxA, Pex-EvxB, Pex-RuntA, Pex-RuntB, Pex-Hes1A, Pex-Hes1B, Pex-Hes4A, and Pex-Hey) found in P. excavatus using semi-quantitative reverse transcription-polymerase chain reaction. Our results indicated these genes showed variable expression during bidirectional regeneration. Six of these genes might play diverse and potentially critical roles in head and/or tail regeneration.
赤子爱胜蚓是一种具有高度双向再生能力的物种。成对规则基因被认为在节肢动物的体节形成中具有祖先功能。然而，环节动物（如 Capitella teleta）中的直系同源基因在外胚层或中胚层中不表现出节段性表达。它们在再生中的作用目前尚不清楚。在这里，我们使用半定量逆转录聚合酶链反应报告了在赤子爱胜蚓中发现的主要成对规则基因（Pex-EvxA、Pex-EvxB、Pex-RuntA、Pex-RuntB、Pex-Hes1A、Pex-Hes1B、Pex-Hes4A 和 Pex-Hey）的表达谱。我们的结果表明，这些基因在双向再生过程中表现出可变表达。其中六个基因可能在头部和/或尾部再生中发挥不同且潜在的关键作用。
