Cho Sung-Jin, Koh Ki Seok, Lee Eun, Park Soon Cheol
Department of Life Science, Chung-Ang University, Seoul, Republic of Korea.
Biosci Biotechnol Biochem. 2009 Dec;73(12):2609-14. doi: 10.1271/bbb.90416. Epub 2009 Dec 7.
The earthworm provides an excellent model for investigating regeneration. Here we report the full-length cloning of three labial genes (Pex-lab01, Pex-lab02, and Pex-lab03) in the earthworm Perionyx excavatus. To analyze their expression pattern during head and tail regeneration, we used the reverse transcription-polymerase chain reaction. Our results indicate that the three labial genes were expressed only in the head-regenerating tissues. Also, we found that the expression of Pex-lab01 and Pex-lab02 is up-regulated, and this indicates their involvement in wound healing and the blastema formation processes during early head regeneration.
蚯蚓为研究再生提供了一个绝佳的模型。在此，我们报告了在赤子爱胜蚓中三个唇基因（Pex-lab01、Pex-lab02和Pex-lab03）的全长克隆。为了分析它们在头部和尾部再生过程中的表达模式，我们使用了逆转录-聚合酶链反应。我们的结果表明，这三个唇基因仅在头部再生组织中表达。此外，我们发现Pex-lab01和Pex-lab02的表达上调，这表明它们参与了早期头部再生过程中的伤口愈合和芽基形成过程。
