Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University Bratislava, Bratislava, Slovakia.
Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University Bratislava, Bratislava, Slovakia.
J Sep Sci. 2024 Oct;47(19):e202400554. doi: 10.1002/jssc.202400554.
The increasing awareness of environmental issues and the transition to green analytical chemistry (GAC) have gained popularity among academia and industry in recent years. One of the principles of GAC is the reduction and replacement of toxic solvents with more sustainable and environmentally friendly ones. This review gives an overview of the advances in applying green solvents as an alternative to the traditional organic solvents for peptide and protein purification and analysis by liquid chromatography (LC) and capillary electrophoresis (CE) methods. The feasibility of using greener LC and CE methods is demonstrated through several application examples; however, there is still plenty of room for new developments to fully realize their potential and to address existing challenges. Thanks to the tunable properties of designer solvents, such as ionic liquids and deep eutectic solvents, and almost infinite possible mixtures of components for their production, it is possible that some new designer solvents could potentially surpass the traditional harmful solvents in the future. Therefore, future research should focus mainly on developing new solvent combinations and enhancing analytical instruments to be able to effectively work with green solvents.
近年来，人们对环境问题的认识不断提高，绿色分析化学（GAC）也越来越受到学术界和工业界的关注。GAC 的原则之一是减少和替代有毒溶剂，转而使用更可持续和环保的溶剂。本综述概述了将绿色溶剂应用于替代传统有机溶剂，用于通过液相色谱（LC）和毛细管电泳（CE）方法对肽和蛋白质进行纯化和分析的进展。通过几个应用实例证明了使用更环保的 LC 和 CE 方法的可行性，但仍有很大的发展空间，以充分发挥其潜力并解决现有挑战。由于设计溶剂（如离子液体和深共熔溶剂）的可调性质，以及几乎无限可能的成分组合来生产它们，因此一些新的设计溶剂有可能在未来超越传统的有害溶剂。因此，未来的研究应主要集中在开发新的溶剂组合和增强分析仪器，以能够有效地使用绿色溶剂。
