含羟基磷灰石、氟化物和生物活性玻璃的配方牙本质再矿化凝胶对牙本质显微硬度的影响：一项研究。

The Effect of Formulated Dentin Remineralizing Gel Containing Hydroxyapatite, Fluoride, and Bioactive Glass on Dentin Microhardness: An Study.

作者信息

Asadi Mohadese, Majidinia Sara, Bagheri Hossein, Hoseinzadeh Melika

机构信息

Dental Materials Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Dental Research Center, Mashhad Dental School, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Int J Dent. 2024 Aug 10;2024:4788668. doi: 10.1155/2024/4788668. eCollection 2024.

DOI:10.1155/2024/4788668

PMID:39376678

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11458271/

Abstract

OBJECTIVES

This study aimed to develop a gel with dentin-remineralizing properties, integrating nano-hydroxyapatite (nHA), sodium fluoride (NaF), and bioactive glass (BG).

MATERIALS AND METHODS

The enamel layer of 40 bovine incisors was removed. The samples were allocated into four groups of 10 each, based on varying concentrations of nHA, BG, and NaF in the gel compositions (wt%): (1) 2.5%-7.5%-0.05%, (2) 5%-5%-0.05%, (3) 7.5%-2.5%-0.05%, and (4) a control group with a base gel lacking remineralizing agents. After 8 hr of demineralization, the dentin surface microhardness was measured at depths of 30, 60, and 140 m. After a 20-day pH cycling, the percentage of surface microhardness recovery (SMHR%) was measured and compared among the groups using the ANOVA and Tukey HSD post hoc tests ( = 0.05). Scanning electron microscopy analysis evaluated each specimen's superficial morphology.

RESULTS

At all depths, the SMHR% of the Group 2 and Group 3 was significantly higher than the control group ( < 0.05). The SMHR% Group 1 (67.39% ± 29.34%) was significantly higher than the control group (-21.24% ± 51.72%) only at the depth of 30 m ( = 0.047). Group 3 had higher SMHR% than Group 2 at all depths; however, the difference was not statistically significant. Moreover, the SMHR% of Group 3 was significantly higher than that of Group 1 at depths of 30 m (187. 94% ± 68.95% vs. 67.39% ± 29.34%; = 0.005) and 60 m (179.55% ± 75.96% vs. 64.34% ± 41.96%; = 0.043). Surface deposition and tubule occlusion were observed in the Groups 2 and 3 samples, which was more prominent in the latter.

CONCLUSIONS

Combining 7.5% nHA, 2.5% BG, and 0.05% NaF could potentially remineralize primary carious lesions.

摘要

目的

本研究旨在开发一种具有牙本质再矿化特性的凝胶，该凝胶整合了纳米羟基磷灰石（nHA）、氟化钠（NaF）和生物活性玻璃（BG）。

材料与方法

去除40颗牛切牙的釉质层。根据凝胶组合物中nHA、BG和NaF的不同浓度（重量百分比），将样本分为四组，每组10个：（1）2.5%-7.5%-0.05%，（2）5%-5%-0.05%，（3）7.5%-2.5%-0.05%，以及（4）一个不含再矿化剂的基础凝胶对照组。脱矿8小时后，在30、60和140μm深度处测量牙本质表面显微硬度。经过20天的pH循环后，测量表面显微硬度恢复百分比（SMHR%），并使用方差分析和Tukey HSD事后检验在各组之间进行比较（α = 0.05）。扫描电子显微镜分析评估每个样本表面形态。

结果

在所有深度处，第2组和第3组的SMHR%均显著高于对照组（P < 0.05）。仅在30μm深度处，第1组的SMHR%（67.39% ± 29.34%）显著高于对照组（-21.24% ± 51.72%）（P = 0.047）。在所有深度处，第3组的SMHR%均高于第2组；然而，差异无统计学意义。此外，在30μm（187.94% ± 68.95%对67.39% ± 29.34%；P = 0.005）和60μm（179.55% ± 75.96%对64.34% ± 41.96%；P = 0.043）深度处，第3组的SMHR%显著高于第1组。在第2组和第3组样本中观察到表面沉积和小管堵塞，在后者中更为明显。