Mizuta Ryosuke, Kanao Eisuke, Ukyo Keigo, Kuwada Shusuke, Sawada Shin-Ichi, Ishihama Yasushi, Akiyoshi Kazunari, Sasaki Yoshihiro
Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Laboratory of Proteomics and Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Nano Lett. 2024 Oct 8. doi: 10.1021/acs.nanolett.4c03293.
Biomembrane coating technologies have been developed to equip synthetic nanomaterials with natural biointerfaces. We report a one-step method for nondestructively coating the biomembranes of "living" cells onto nanoparticle surfaces. By using simple centrifugation, nanoparticles pass through a concentrated layer of living cells. This process mimics exosome release via endocytosis and exocytosis, preserving the membrane integrity of the source cells. The resulting silica nanoparticles were efficiently coated with membrane components from Raw264.7 cells. Nanoflow-liquid chromatography-tandem mass spectrometry confirmed that the proteins composing the membrane originated from the source cells. Additionally, the biomembrane coating suppressed the phagocytosis of silica nanoparticles by Raw264.7 cells while enhancing their uptake by HeLa cells. Our simple and efficient method for living biomembrane coating holds promise for the development of nanoparticles for medical and pharmaceutical applications.
生物膜涂层技术已被开发用于为合成纳米材料赋予天然生物界面。我们报告了一种将“活”细胞的生物膜无损涂覆到纳米颗粒表面的一步法。通过简单的离心，纳米颗粒穿过活细胞的浓缩层。这个过程模拟了通过内吞作用和外排作用释放外泌体，保留了源细胞的膜完整性。所得的二氧化硅纳米颗粒被来自Raw264.7细胞的膜成分有效包覆。纳流液相色谱-串联质谱证实构成膜的蛋白质源自源细胞。此外，生物膜涂层抑制了Raw264.7细胞对二氧化硅纳米颗粒的吞噬作用，同时增强了HeLa细胞对它们的摄取。我们这种简单有效的活生物膜涂覆方法有望用于开发用于医学和制药应用的纳米颗粒。
