Department of Microbial Pathogenesis, University of Maryland - Baltimore, Baltimore, Maryland, USA.
Medical Scientist Training Program, University of Maryland - Baltimore, Baltimore, Maryland, USA.
Microbiol Spectr. 2024 Nov 5;12(11):e0053024. doi: 10.1128/spectrum.00530-24. Epub 2024 Oct 8.
Diffuse panbronchiolitis (DPB) is a rare, idiopathic inflammatory disease primarily diagnosed in East Asian populations. DPB is characterized by diffuse pulmonary lesions, inflammation of the respiratory bronchioles, and bacterial infections of the airway. Historically, sputum cultures reveal in 22% of DPB patients, increasing to 60% after 4 years from disease onset. Although DPB patients have a known susceptibility to respiratory infections, as is observed in other chronic lung diseases such as cystic fibrosis (CF), the characterization of DPB strains is limited. In this study, we characterized 24 strains obtained from a cohort of DPB patients for traits previously associated with virulence, including growth, motility, antibiotic susceptibility, lipopolysaccharide structure, and genomic diversity. Our cohort of DPB strains exhibits considerable genomic variability when compared with isolates from people with cystic fibrosis chronically colonized with and acute infection isolates. Similar to CF, DPB strains produce a diverse array of modified lipid A structures. Antibiotic susceptibility testing revealed increased resistance to erythromycin, a representative agent of the macrolide antibiotics used to manage DPB patients. Differences in the O-antigen type among strains collected from these different backgrounds were also observed. Ultimately, the characterization of DPB strains highlights several unique qualities of strains collected from chronically diseased airways, underscoring the challenges in treating DPB, CF, and other obstructive respiratory disease patients with infections.
Diffuse panbronchiolitis (DPB), a chronic lung disease characterized by persistent infection, serves as an informative comparator to more common chronic lung diseases, such as cystic fibrosis (CF). This study aimed to better address the interplay between and chronically compromised airway environments through the examination of DPB strains, as existing literature regarding DPB is limited to case reports, case series, and clinical treatment guidelines. The evaluation of these features in the context of DPB, in tandem with prevailing knowledge of strains collected from more common chronic lung diseases (e.g., CF), can aid in the development of more effective strategies to combat respiratory infections in patients with chronic lung diseases.
弥漫性泛细支气管炎（DPB）是一种罕见的特发性炎症性疾病，主要在东亚人群中诊断。DPB 的特征是弥漫性肺部病变、呼吸细支气管炎症和气道细菌感染。历史上，痰培养在 22%的 DPB 患者中发现，从发病后 4 年增加到 60%。尽管 DPB 患者已知易患呼吸道感染，就像在其他慢性肺部疾病如囊性纤维化（CF）中观察到的那样，但 DPB 菌株的特征有限。在这项研究中，我们对来自 DPB 患者队列的 24 株菌株进行了特征描述，这些菌株与先前与毒力相关的特征有关，包括生长、运动性、抗生素敏感性、脂多糖结构和基因组多样性。与囊性纤维化患者慢性定植和急性感染分离株相比，我们的 DPB 菌株队列表现出相当大的基因组变异性。与 CF 相似，DPB 菌株产生多样化的修饰脂 A 结构。抗生素敏感性测试显示，对红霉素的耐药性增加，红霉素是用于治疗 DPB 患者的大环内酯类抗生素的代表药物。从这些不同背景中收集的菌株的 O-抗原型也存在差异。最终，DPB 菌株的特征强调了从慢性疾病气道中收集的菌株的几个独特品质，突出了治疗 DPB、CF 和其他阻塞性呼吸道疾病患者感染的挑战。
弥漫性泛细支气管炎（DPB）是一种以持续感染为特征的慢性肺部疾病，可作为更常见的慢性肺部疾病（如囊性纤维化（CF））的信息比较器。本研究旨在通过检查 DPB 菌株来更好地解决与慢性气道受损环境之间的相互作用，因为关于 DPB 的现有文献仅限于病例报告、病例系列和临床治疗指南。在 DPB 的背景下评估这些特征，与从更常见的慢性肺部疾病（如 CF）中收集的菌株的现有知识相结合，可以帮助制定更有效的策略来对抗慢性肺部疾病患者的呼吸道感染。
