Bacterial Hybrid Light-Emitting Diodes.

Photon down-converting filters with fluorescent proteins (FPs) are a new frontier in the quest for rare-earth-free and non-toxic color filters for white light-emitting diodes. There are, however, concerns related to the FP purification costs and lack of FP recyclability/reuse. Here, the direct use of bacteria in photon down-converting filters can be of utmost relevance, eliminating purification and allowing in situ production of new FPs. However, their high background autofluorescence/scattering and low stability in polymer coatings have traditionally hampered the application of Engineering Living Materials (ELMs) for photon manipulation. Indeed, there are no examples of ELMs in lighting systems. This work discloses the first protocol to prepare living spheroplasts with > 90% scattering reduction, high FP expression fairly keeping their photoluminescence figures-of-merit, and excellent resilience in polymer films over 1 year under ambient storage. This unlocked the preparation of the first bacteria hybrid light-emitting diodes integrating ELMs for photon conversion. These devices feature similar stabilities to those using purified FPs, while enabling a cost-effective strategy and active FP recycling by the simple recultivation of spheroplasts. Overall, this work introduces a successful case toward bacteria-polymer photon manipulation, in general, and a new living lighting concept, in particular.