Department of Microbiology, Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences, Thandalam, Tamilnadu, India.
Department of Botany and Microbiology, College of Science, King Saud University, P.O.Box 2455, Riyad, 11451, Saudi Arabia.
Microb Pathog. 2024 Nov;196:106998. doi: 10.1016/j.micpath.2024.106998. Epub 2024 Oct 9.
Pseudomonas aeruginosa (P. aeruginosa), a Gram-negative opportunistic pathogen, poses significant treatment challenges due to its antibiotic resistance and biofilm formation. This study investigates the anti-bacterial and anti-biofilm activities of chemically synthesized selenium nanoparticles (SeNPs) against P. aeruginosa. SeNPs were synthesized using ascorbic acid as a reducing agent and characterized. Biofilm formation was quantified using a modified microtiter plate method, and the anti-biofilm efficacy of SeNPs was evaluated using confocal microscopy and SEM. The P. aeruginosa isolates exhibited high resistance to piperacillin-tazobactam (60 %) and ceftazidime (59 %). SeNPs demonstrated a round shape with a diameter of 15-18 nm. UV-Vis spectra showed a peak at 275 nm, and XRD analysis revealed crystalline peaks corresponding to selenium. The FTIR spectra confirmed the presence of various functional groups. SeNPs significantly reduced biofilm formation in a dose-dependent manner, with MIC50 and MIC90 values of 60 μg/mL and 80 μg/mL, respectively. Confocal microscopy and SEM analysis showed a notable decrease in biofilm thickness and bacterial adherence post-SeNPs treatment. These findings suggest that SeNPs could be a promising alternative or adjunctive treatment option for combating antibiotic-resistant P. aeruginosa infections. Further research is warranted to explore the clinical applications of SeNPs in treating biofilm-associated infections.
铜绿假单胞菌（P. aeruginosa）是一种革兰氏阴性机会致病菌，由于其对抗生素的耐药性和生物膜的形成，给治疗带来了巨大的挑战。本研究旨在探讨化学合成的硒纳米粒子（SeNPs）对铜绿假单胞菌的抗菌和抗生物膜活性。使用抗坏血酸作为还原剂合成 SeNPs 并进行了表征。采用改良微量平板法定量测定生物膜形成，并用共聚焦显微镜和扫描电子显微镜评估 SeNPs 的抗生物膜效果。分离的铜绿假单胞菌对哌拉西林-他唑巴坦（60%）和头孢他啶（59%）表现出高度耐药性。SeNPs 呈圆形，直径为 15-18nm。紫外-可见光谱在 275nm 处有一个峰值，XRD 分析显示出与硒相对应的结晶峰。傅里叶变换红外光谱证实存在各种官能团。SeNPs 以剂量依赖的方式显著减少生物膜形成，MIC50 和 MIC90 值分别为 60μg/mL 和 80μg/mL。共聚焦显微镜和 SEM 分析显示，生物膜厚度和细菌黏附在 SeNPs 处理后明显减少。这些发现表明，SeNPs 可能是治疗抗药性铜绿假单胞菌感染的一种有前途的替代或辅助治疗选择。需要进一步的研究来探索 SeNPs 在治疗生物膜相关感染中的临床应用。
