多花菝葜（何首乌）和多花菝葜变种狭叶菝葜的 T2T 基因组组装。
T2T genome assemblies of Fallopia multiflora (Heshouwu) and F. multiflora var. angulata.
机构信息
Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China, South China Botanical Garden, Chinese Academy of Sciences, South China National Botanical Garden, Guangzhou, 510650, P.R. China.
College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi, 341000, P.R. China.
出版信息
Sci Data. 2024 Oct 9;11(1):1103. doi: 10.1038/s41597-024-03943-4.
The traditional Chinese medicinal plant Fallopia multiflora (hereafter AYY) is well known for its anti-hyperlipidaemia, immunomodulating, and hepatoprotective effects, attributed to its abundance of anthraquinones and stilbene glycosides, which are distinct to its variety F. multiflora var. angulata (hereafter CYY) in proportion and composition. In this study, telomere-to-telomere (T2T) genomes were assembled for AYY and CYY using PacBio HiFi reads and Hi-C data. The genome sizes, percentages of repetitive sequences, and numbers of protein-coding genes of AYY and CYY assemblies were 1,458.37 Mb/70.48%/84,768 and 1,174.38 Mb/67.36%/69,100, respectively. Comprehensive assessments confirmed high continuity (contig N50: 112.58 Mb and 94.83 Mb; number of gaps: 9 and 5), completeness (BUSCOs: 97.30% and 97.60%; LAI: 16.93 and 16.77), and correctness (QV: 51.42 and 52.60) of AYY and CYY assemblies. These T2T genomes of F. multiflora provide valuable resources for studying the biosynthesis of specialized metabolites and facilitating precise genetic improvement.
传统中药何首乌（以下简称 AYY）以其抗高血脂、免疫调节和保肝作用而闻名，这归因于其丰富的蒽醌和二苯乙烯苷，其比例和组成与品种 F. multiflora var. angulata（以下简称 CYY）不同。在这项研究中，使用 PacBio HiFi 读取和 Hi-C 数据为 AYY 和 CYY 组装端到端（T2T）基因组。AYY 和 CYY 组装体的基因组大小、重复序列百分比和蛋白质编码基因数分别为 1,458.37 Mb/70.48%/84,768 和 1,174.38 Mb/67.36%/69,100。综合评估证实了 AYY 和 CYY 组装体具有高连续性（contig N50：112.58 Mb 和 94.83 Mb；gap 数量：9 和 5）、完整性（BUSCOs：97.30% 和 97.60%；LAI：16.93 和 16.77）和正确性（QV：51.42 和 52.60）。这些 F. multiflora 的 T2T 基因组为研究特殊代谢物的生物合成和促进精确遗传改良提供了有价值的资源。
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