Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
South China National Botanical Garden, Guangzhou, 510650, China.
Sci Data. 2023 Sep 16;10(1):631. doi: 10.1038/s41597-023-02546-9.
Musa acuminata is a main wild contributor to banana cultivars. Here, we reported a haplotype-resolved and telomere-to-telomere reference genome of M. acuminata by incorporating PacBio HiFi reads, Nanopore ultra-long reads, and Hi-C data. The genome size of the two haploid assemblies was estimated to be 469.83 Mb and 470.21 Mb, respectively. Multiple assessments confirmed the contiguity (contig N50: 16.53 Mb and 18.58 Mb; LAI: 20.18 and 19.48), completeness (BUSCOs: 98.57% and 98.57%), and correctness (QV: 45.97 and 46.12) of the genome. The repetitive sequences accounted for about half of the genome size. In total, 40,889 and 38,269 protein-coding genes were annotated in the two haploid assemblies, respectively, of which 9.56% and 3.37% were newly predicted. Genome comparison identified a large reciprocal translocation involving 3 Mb and 10 Mb from chromosomes 01 and 04 within M. acuminata. This reference genome of M. acuminata provides a valuable resource for further understanding of subgenome evolution of Musa species, and precise genetic improvement of banana.
芭蕉属是香蕉栽培品种的主要野生祖先之一。在这里，我们通过整合 PacBio HiFi reads、Nanopore 超长reads 和 Hi-C 数据，报道了一个单倍型解析和端粒到端粒的 Musa acuminata 参考基因组。两个单倍体组装的基因组大小估计分别为 469.83 Mb 和 470.21 Mb。多种评估方法证实了基因组的连续性（contig N50：16.53 Mb 和 18.58 Mb；LAI：20.18 和 19.48）、完整性（BUSCOs：98.57% 和 98.57%）和正确性（QV：45.97 和 46.12）。重复序列约占基因组大小的一半。在两个单倍体组装中共注释了 40889 和 38269 个蛋白质编码基因，其中分别有 9.56%和 3.37%是新预测的。基因组比较鉴定出一个涉及 01 号和 04 号染色体的 3 Mb 和 10 Mb 的大规模相互易位。Musa acuminata 的这个参考基因组为进一步了解芭蕉属物种的亚基因组进化以及香蕉的精确遗传改良提供了有价值的资源。
