College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.
Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.
J Agric Food Chem. 2024 Oct 23;72(42):23520-23532. doi: 10.1021/acs.jafc.4c07166. Epub 2024 Oct 10.
Insecticides are commonly utilized in agriculture and forestry for pest control, but their dispersal can pose hazards to humans and environment. Understanding resistance, inheritance patterns, and fitness costs can help manage resistance. A λ-cyhalothrin-resistant population (LCR) of , a global pest of pome fruits and walnuts, was obtained through selective insecticide breeding for 15 generations, showing stable moderate resistance (23.85-fold). This population was cross-resistant to deltamethrin (4.26-fold) but not to β-cypermethrin, chlorantraniliprole, chlorpyrifos, and avermectin. Genetic analysis revealed the resistance was autosomal, incompletely dominant, and controlled by multiple genes. Increased activity of glutathione S-transferases and cytochrome P450 monooxygenases (P450s) played a primary role in resistance, with specific genes up-regulated in LCR, and exhibited significant expression in midgut. LCR also exhibited fitness costs, including delays in development, reduced fecundity, and slower population growth. These findings contribute to understanding λ-cyhalothrin resistance in and can guide resistance management strategies.
杀虫剂通常用于农业和林业中的害虫防治，但它们的扩散可能对人类和环境造成危害。了解抗性、遗传模式和适合度代价有助于管理抗性。通过对 15 代选择性杀虫剂选育，获得了一种全球苹果和核桃害虫 的 λ-氯氰菊酯抗性种群（LCR），表现出稳定的中度抗性（23.85 倍）。该种群对溴氰菊酯（4.26 倍）具有交叉抗性，但对β-氯氰菊酯、氯虫苯甲酰胺、毒死蜱和阿维菌素无抗性。遗传分析表明，抗性是常染色体、不完全显性的，由多个基因控制。谷胱甘肽 S-转移酶和细胞色素 P450 单加氧酶（P450s）活性的增加在抗性中起主要作用，LCR 中特定基因上调，并在中肠中表现出显著表达。LCR 还表现出适合度代价，包括发育延迟、繁殖力降低和种群增长缓慢。这些发现有助于了解 中 λ-氯氰菊酯的抗性，并可以指导抗性管理策略。
