Nunn Brook L, Timmins-Schiffman Emma, Mudge Miranda C, Plubell Deanna, Chebli Gabriella, Kubanek Julia, Riffle Michael, Noble William S, Harvey Elizabeth, Nunn Tasman A, Huntemann Marcel, Clum Alicia, Foster Brian, Foster Bryce, Roux Simon, Palaniappan Krishnaveni, Mukherjee Supratim, Reddy T B K, Daum Chris, Copeland Alex, Chen I-Min A, Ivanova Natalia N, Kyrpides Nikos C, Glavina Del Rio Tijana, Eloe-Fadrosh Emiley A
University of Washington, Department of Genome Sciences, Seattle, WA 98195.
Georgia Institute of Technology, School of Biological Sciences and School of Chemistry & Biochemistry, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta, GA 30332.
bioRxiv. 2024 Sep 28:2024.09.27.614549. doi: 10.1101/2024.09.27.614549.
In May and June of 2021, marine microbial samples were collected for DNA sequencing in East Sound, WA, USA every 4 hours for 22 days. This high temporal resolution sampling effort captured the last 3 days of a sp. bloom, the initiation and complete bloom cycle of (8 days), and the following bacterial bloom (2 days). Metagenomes were completed on the time series, and the dataset includes 128 size-fractionated microbial samples (0.22-1.2 μm), providing gene abundances for the dominant members of bacteria, archaea, and viruses. This dataset also has time-matched nutrient analyses, flow cytometry data, and physical parameters of the environment at a single point of sampling within a coastal ecosystem that experiences regular bloom events, facilitating a range of modeling efforts that can be leveraged to understand microbial community structure and their influences on the growth, maintenance, and senescence of phytoplankton blooms.
2021年5月和6月，在美国华盛顿州东桑德，每4小时采集一次海洋微生物样本进行DNA测序，共持续22天。这种高时间分辨率的采样工作捕捉到了一种甲藻藻华的最后3天、另一种藻（8天）的起始和完整藻华周期，以及随后的细菌藻华（2天）。对该时间序列完成了宏基因组测序，数据集包括128个大小分级的微生物样本（0.22 - 1.2μm），提供了细菌、古菌和病毒主要成员的基因丰度。该数据集还具有时间匹配的营养分析、流式细胞术数据以及在一个经常发生藻华事件的沿海生态系统内单个采样点的环境物理参数，有助于开展一系列建模工作，以了解微生物群落结构及其对浮游植物藻华生长、维持和衰老的影响。
