Li Haimei, Tan Xinjia, Qin Liyan, Gatasheh Mansour K, Zhang Lei, Lin Wenmin, Hu Feng, Yan Rian, Alshammri Mariam K, Shen Yingbin, Abbasi Arshad Mehmood, Qi Jing
School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Longping Branch, College of Biology, Hunan University, Changsha, 410125, China.
Heliyon. 2024 Sep 21;10(19):e38283. doi: 10.1016/j.heliyon.2024.e38283. eCollection 2024 Oct 15.
Corosolic acid (CA), a pentacyclic triterpenoid, exhibits remarkably low hydrophilicity, restricting its application in aqueous systems. To enhance its hydrophilicity, we optimised nanoemulsion preparation conditions, resulting in a stable corosolic acid nanoemulsion system. By screening the oil phase, surfactant, and cosurfactant, along with investigating the mass ratio of surfactant and cosurfactant and the preparation temperature, we achieved an optimal corosolic acid nanoemulsion. We measured the particle size, polydispersity coefficient, and Zeta potential of the optimised formulation. The nanoemulsion's sustained-release effect, stability, and antibacterial activity were subsequently examined. The optimised formulation comprised ethyl oleate, cremophor EL, and Tween 80 (1.5:1), combined with ethanol in a ratio of 1:9:2.25 (w/w/w), and was prepared at 30 °C. This optimised corosolic acid nanoemulsion exhibited uniform particle size distribution, favourable dispersion, and notable slow-release capabilities. Importantly, the nanoemulsion demonstrated exceptional stability. In comparison to the positive control's bacteriostatic zone diameter, it was evident that the CA nanoemulsion (1.06 ± 0.11 mm) and blank nanoemulsion (1.03 ± 0.05 mm) both displayed notable inhibitory activity against . Our findings established a solid foundation for the potential application of CA nanoemulsion in the food, cosmetics, and pharmaceutical industries. However, the application of CA nanoemulsion in real food or drug systems has not been explored yet.
熊果酸（CA）是一种五环三萜类化合物，亲水性极低，限制了其在水性体系中的应用。为提高其亲水性，我们优化了纳米乳液的制备条件，得到了稳定的熊果酸纳米乳液体系。通过筛选油相、表面活性剂和助表面活性剂，并研究表面活性剂与助表面活性剂的质量比以及制备温度，我们制备出了最优的熊果酸纳米乳液。我们测量了优化配方的粒径、多分散系数和Zeta电位。随后考察了纳米乳液的缓释效果、稳定性和抗菌活性。优化后的配方包含油酸乙酯、聚氧乙烯蓖麻油EL和吐温80（1.5:1），与乙醇的比例为1:9:2.25（w/w/w），在30℃下制备。这种优化后的熊果酸纳米乳液粒径分布均匀，分散性良好，具有显著的缓释能力。重要的是，该纳米乳液表现出优异的稳定性。与阳性对照的抑菌圈直径相比，很明显CA纳米乳液（1.06±0.11mm）和空白纳米乳液（1.03±0.05mm）对……均表现出显著的抑制活性。我们的研究结果为CA纳米乳液在食品、化妆品和制药行业的潜在应用奠定了坚实基础。然而，CA纳米乳液在实际食品或药物体系中的应用尚未得到探索。
