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大分子结晶高级方法实践课程：20年历程与未来展望

Practical courses on advanced methods in macromolecular crystallization: 20 years of history and future perspectives.

作者信息

Havlickova Petra, Gavira Jose A, Mesters Jeroen R, Koutska Anna, Kascakova Barbora, Prudnikova Tatyana, Hilgenfeld Rolf, Garcia-Ruiz Juan Manuel, Rezacova Pavlina, Kuta Smatanova Ivana

机构信息

Department of Chemistry, Faculty of Science University of South Bohemia in Ceske Budejovice Branisovska 1760 37005Ceske Budejovice Czechia.

Laboratorio de Estudios Cristalográficos Instituto Andaluz de Ciencias de la Tierra (CSIC) Avenida de las Palmeras 4 18100Armilla Granada Spain.

出版信息

J Appl Crystallogr. 2024 Aug 30;57(Pt 5):1609-1617. doi: 10.1107/S1600576724007106. eCollection 2024 Oct 1.

DOI:10.1107/S1600576724007106
PMID:39387072
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11460401/
Abstract

The first Federation of European Biochemical Societies Advanced Course on macromolecular crystallization was launched in the Czech Republic in October 2004. Over the past two decades, the course has developed into a distinguished event, attracting students, early career postdoctoral researchers and lecturers. The course topics include protein purification, characterization and crystallization, covering the latest advances in the field of structural biology. The many hands-on practical exercises enable a close interaction between students and teachers and offer the opportunity for students to crystallize their own proteins. The course has a broad and lasting impact on the scientific community as participants return to their home laboratories and act as nuclei by communicating and implementing their newly acquired knowledge and skills.

摘要

欧洲生物化学学会联合会首届大分子结晶高级课程于2004年10月在捷克共和国开办。在过去二十年里，该课程已发展成为一项卓越的活动，吸引了学生、处于职业生涯早期的博士后研究人员和讲师。课程主题包括蛋白质纯化、表征和结晶，涵盖了结构生物学领域的最新进展。众多的实践操作练习使学生与教师之间能够密切互动，为学生提供了结晶自己蛋白质的机会。该课程对科学界产生了广泛而持久的影响，因为参与者回到自己的实验室后，会通过交流和应用新获得的知识与技能发挥核心作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/9fb4ec4db076/j-57-01609-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/f48ed55f564e/j-57-01609-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/59ed456f20fd/j-57-01609-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/0c32b952dfe9/j-57-01609-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/64a0fc724428/j-57-01609-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/f8a13eef3e63/j-57-01609-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/1b03532a090b/j-57-01609-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/9ef93c496eea/j-57-01609-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/9fb4ec4db076/j-57-01609-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/f48ed55f564e/j-57-01609-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/59ed456f20fd/j-57-01609-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/0c32b952dfe9/j-57-01609-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/64a0fc724428/j-57-01609-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/f8a13eef3e63/j-57-01609-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/1b03532a090b/j-57-01609-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/9ef93c496eea/j-57-01609-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f098/11460401/9fb4ec4db076/j-57-01609-fig8.jpg

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A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.一种用于通过串行晶体学实现高效生物大分子结晶和结构分析的简单通用微流控装置。
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Combining crystallogenesis methods to produce diffraction-quality crystals of a psychrophilic tRNA-maturation enzyme.
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Acta Crystallogr F Struct Biol Commun. 2018 Nov 1;74(Pt 11):747-753. doi: 10.1107/S2053230X18014590. Epub 2018 Oct 31.
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Crystallographic education in the 21st century.21世纪的晶体学教育。
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