马冠状病毒在马肠类器官中的感染和复制。
Equine coronavirus infection and replication in equine intestinal enteroids.
机构信息
Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
Division of Zoonosis Research, Sapporo Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-0045, Japan.
出版信息
Vet Res. 2024 Oct 10;55(1):135. doi: 10.1186/s13567-024-01381-z.
In this study, equine intestinal enteroids (EIEs) were generated from the duodenum, jejunum, and ileum and inoculated with equine coronavirus (ECoV) to investigate their suitability as in vitro models with which to study ECoV infection. Immunohistochemistry revealed that the EIEs were composed of various cell types expressed in vivo in the intestinal epithelium. Quantitative reverse-transcription PCR (qRT-PCR) and virus titration showed that ECoV had infected and replicated in the EIEs. These results were corroborated by electron microscopy. This study suggests that EIEs can be novel in vitro tools for studying the interaction between equine intestinal epithelium and ECoV.
在这项研究中，从马的十二指肠、空肠和回肠中生成了肠类器官（EIEs），并用马冠状病毒（ECoV）接种，以研究它们作为体外模型研究 ECoV 感染的适用性。免疫组织化学显示，EIEs 由在肠上皮细胞中体内表达的各种细胞类型组成。定量逆转录 PCR（qRT-PCR）和病毒滴定表明，ECoV 已感染并在 EIEs 中复制。电子显微镜证实了这些结果。这项研究表明，EIEs 可以成为研究马肠道上皮细胞与 ECoV 相互作用的新型体外工具。
Zotero 插件