Kumar Mukesh, Sachan Rohan Samir Kumar, Kauts Simran, Karnwal Arun, Mahmoud Alaa El Din
Department of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, India.
Department of Zoology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, India.
Environ Technol. 2025 Apr;46(11):1909-1921. doi: 10.1080/09593330.2024.2409994. Epub 2024 Oct 10.
Polyhydroxybutyrate (PHB) is considered as a hope for bioplastic production, which can serve as a sustainable alternative. Utilizing feedstock as substrate is widely explored for the production but wood waste, which is abundant in cellulose, hemicellulose and lignocellulose, has limited studies for PHB production. Herein, wood waste is used as a biobased feedstock Hydrolyses of wood waste was done using sulphuric acid (HSO) to break down of cellulose and hemicellulose into simple carbon forms. The hydrolysed product was analysed for sugar presence by quantitative and qualitative methods. bacterial strain was used for the production purpose using hydrolysed wood waste as substrate media. The Plackett-Burman design (PBD) and response surface methodology (RSM) were applied to optimize the growth media. The results of PBD were used to identify significant factors influencing PHB production, which were then further optimized using RSM. The work's results conclusively demonstrated that . possesses the capability to effectively utilize wood waste and wastewater as substrate media up to production rate of 13-14 mg mL of PHB. Fourier Transformed Infra-Red (FTIR) spectroscopic peaks confirm the produced product is PHB, which is a type of polyhydroxyalkanoate (PHA), classified within the polyester family highlighting wood waste potential as a sustainable solution to address plastic pollution.
聚羟基丁酸酯（PHB）被视为生物塑料生产的希望所在，它可作为一种可持续的替代品。利用原料作为底物进行PHB生产已得到广泛探索，但富含纤维素、半纤维素和木质纤维素的木材废料用于PHB生产的研究却很有限。在此，木材废料被用作生物基原料。使用硫酸（H₂SO₄）对木材废料进行水解，以将纤维素和半纤维素分解为简单的碳形式。通过定量和定性方法对水解产物进行糖含量分析。使用水解后的木材废料作为底物培养基，采用一种细菌菌株进行生产。应用Plackett-Burman设计（PBD）和响应面方法（RSM）来优化生长培养基。PBD的结果用于确定影响PHB生产的显著因素，然后使用RSM进一步优化这些因素。该工作的结果最终表明，[细菌名称未给出]能够有效利用木材废料和废水作为底物培养基，PHB的生产率可达13 - 14毫克/毫升。傅里叶变换红外（FTIR）光谱峰证实所生产的产物是PHB，它是聚羟基脂肪酸酯（PHA）的一种，属于聚酯家族，凸显了木材废料作为解决塑料污染的可持续解决方案的潜力。
