Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC, 29631, USA.
Mol Genet Genomics. 2024 Oct 12;299(1):97. doi: 10.1007/s00438-024-02179-6.
The food industry has incurred substantial losses from contamination by Pseudomonas fluorescens, emphasizing the critical importance of implementing effective control strategies. Phages are potential sterilizers due to their specific killing abilities and the difficulty bacteria face in developing resistance. However, a significant barrier to their development is the lack of diversity among phage types. In this study, we characterized a novel lytic P. fluorescens phage, named vB_PF_Y1-MI. Phage vB_PF_Y1-MI displayed a latent period of nearly 10 min and a high burst size of 1493 PFU/cell. This phage showed good activity over a wide range of temperature (up to 70 °C) and pH (3-12). The genome of phage vB_PF_Y1-MI spans 93,233 bp with a GC content of 45%. It encompasses 174 open-reading frames and 19 tRNA genes, while no lysogeny or virulence-associated genes were detected. Phylogenetic analysis positions it as a novel unassigned evolutionary lineage within the Caudoviricetes class among related dsDNA phages. Our study provides foundational insights into vB_PF_Y1-MI and emphasizes its potential as an effective biological control agent against P. fluorescens. This research offers crucial theoretical groundwork and technical support for subsequent efforts in preventing and controlling P. fluorescens contamination.
食品工业因荧光假单胞菌污染而遭受重大损失，这强调了实施有效控制策略的至关重要性。噬菌体由于其特定的杀菌能力和细菌难以产生抗性而成为潜在的杀菌剂。然而，它们发展的一个重大障碍是噬菌体类型的多样性缺乏。在本研究中，我们对一种新型溶菌荧光假单胞菌噬菌体进行了表征，命名为 vB_PF_Y1-MI。噬菌体 vB_PF_Y1-MI 的潜伏期接近 10 分钟，爆发量高达 1493PFU/细胞。该噬菌体在很宽的温度（高达 70°C）和 pH 值（3-12）范围内都具有很好的活性。噬菌体 vB_PF_Y1-MI 的基因组跨度为 93233bp，GC 含量为 45%。它包含 174 个开放阅读框和 19 个 tRNA 基因，而未检测到溶原或毒力相关基因。系统发育分析将其定位为相关双链 DNA 噬菌体中 Caudoviricetes 类中的一个新的未分类进化谱系。本研究为 vB_PF_Y1-MI 提供了基础见解，并强调了其作为荧光假单胞菌有效生物控制剂的潜力。这项研究为后续预防和控制荧光假单胞菌污染提供了重要的理论基础和技术支持。
