对荧光假单胞菌DSM 50090具有裂解活性的新型烈性噬菌体PMBT14的基因组序列
Genome sequence of the novel virulent bacteriophage PMBT14 with lytic activity against Pseudomonas fluorescens DSM 50090.
作者信息
Koberg Sabrina, Gieschler Stefanie, Brinks Erik, Wenning Mareike, Neve Horst, Franz Charles M A P
机构信息
Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Hermann-Weigmann-Str. 1, 24103, Kiel, Germany.
Lehrstuhl für Mikrobielle Ökologie, ZIEL-Institute for Food and Health, Technische Universität München, Weihenstephaner Berg 3, 85354, Freising, Germany.
出版信息
Arch Virol. 2018 Sep;163(9):2575-2577. doi: 10.1007/s00705-018-3882-y. Epub 2018 May 21.
Psychrotrophic gram-negative Pseudomonas spp. represent a serious problem in the dairy industry as they can cause spoilage of milk and dairy products. Bacteriophages have moved into focus as promising biocontrol agents for such food spoilage bacteria. The virulent Siphoviridae phage PMBT14 was isolated on a mutant variant of P. fluorescens DSM 50090 challenged with an unrelated virulent P. fluorescens DSM 50090 Podoviridae phage (i.e., mutant strain DSM 50090). PMBT14 has a 47,820-bp dsDNA genome with 76 predicted open reading frames (ORFs). Its genome shows no significant sequence similarity to that of known phages, suggesting that PMBT14 represents a novel phage. Phage PMBT14 could be a promising biocontrol agent for P. fluorescens in milk or dairy foods.
嗜冷革兰氏阴性假单胞菌属在乳制品行业中是一个严重问题，因为它们会导致牛奶和乳制品变质。噬菌体作为针对此类食品腐败细菌的有前景的生物防治剂已成为关注焦点。烈性长尾噬菌体科噬菌体PMBT14是在荧光假单胞菌DSM 50090的一个突变变体上分离得到的，该突变变体受到一种无关的烈性荧光假单胞菌DSM 50090短尾噬菌体科噬菌体（即突变株DSM 50090）的攻击。PMBT14有一个47,820碱基对的双链DNA基因组，带有76个预测的开放阅读框（ORF）。其基因组与已知噬菌体的基因组没有显著的序列相似性，这表明PMBT14代表一种新型噬菌体。噬菌体PMBT14可能是用于牛奶或乳制品中荧光假单胞菌的一种有前景的生物防治剂。
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