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新型T7样荧光假单胞菌噬菌体PFP1的全基因组序列

Complete genome sequence of PFP1, a novel T7-like Pseudomonas fluorescens bacteriophage.

作者信息

Li Meng, Chen Xinran, Ma Yongsheng, Li Zhibo, Zhao Qiancheng

机构信息

College of Food Science and Engineering, Dalian Ocean University, Dalian, 116023, China.

出版信息

Arch Virol. 2018 Dec;163(12):3423-3426. doi: 10.1007/s00705-018-3979-3. Epub 2018 Aug 17.

Abstract

The complete genomic sequence of Pseudomonas fluorescens bacteriophage PFP1, isolated from sewage samples collected in Liaoning Province, China, were sequenced in this study and found to be 40,914 bp long. The PFP1 genome is composed of linear double-stranded DNA with 55.81% G+C content and 45 putative protein-coding genes, and no rRNA and tRNA genes. Comparative genomics and phylogenetic analysis revealed that the Pseudomonas fluorescens phage PFP1 is a new member of the genus T7virus. This information can be used to develop novel phage-based control strategies against Pseudomonas fluorescens.

摘要

本研究对从中国辽宁省采集的污水样本中分离出的荧光假单胞菌噬菌体PFP1的完整基因组序列进行了测序,发现其长度为40,914 bp。PFP1基因组由线性双链DNA组成,G+C含量为55.81%,有45个推定的蛋白质编码基因,无rRNA和tRNA基因。比较基因组学和系统发育分析表明,荧光假单胞菌噬菌体PFP1是T7virus属的一个新成员。该信息可用于开发针对荧光假单胞菌的新型噬菌体控制策略。

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