He Tao-Bin, Bao Yu, Liu Hong-Jun, Jiang Jia-Nan, Jiang Guo Dong, Xu De Hong, Shen Xiao-Jiang, Yang Qing-Song, Hu Jiang-Miao
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People's Republic of China.
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People's Republic of China; College of Ethnic Medicine, Yunnan Minzu University, Kunming 650000, Yunnan, People's Republic of China.
Int J Biol Macromol. 2024 Nov;281(Pt 3):136413. doi: 10.1016/j.ijbiomac.2024.136413. Epub 2024 Oct 10.
Based on structural elucidation of natural and hydrolyzed glycans, the general glycans profiling of D. officinale were unequivocally established for the first time as follows: The results indicated that the structure of D. officinale glycans with low degree of polymerization (DP ≤ 22) was linear α-D-1,4-glucan, whereas the structure of glycans with high degree of polymerization (DP > 24) was linear acetylated 1,4-glucomannan. The content of acetyl groups and mannose to glucose (M/G) ratio increased with the degree of polymerization of D. officinale glycans. In addition, this study showed that natural D. officinale glycans protected pancreatic β-cell damage induced by glucotoxicity through the extracellular signal-regulated kinase (ERK)1/2 pathway.
基于对天然和水解聚糖的结构解析，首次明确建立了铁皮石斛的一般聚糖谱，结果如下：结果表明，低聚合度（DP≤22）的铁皮石斛聚糖结构为线性α-D-1,4-葡聚糖，而高聚合度（DP>24）的聚糖结构为线性乙酰化1,4-葡甘露聚糖。乙酰基含量和甘露糖与葡萄糖（M/G）比值随铁皮石斛聚糖聚合度的增加而增加。此外，本研究表明，天然铁皮石斛聚糖通过细胞外信号调节激酶（ERK）1/2途径保护由糖毒性诱导的胰腺β细胞损伤。
