Laboratory of Pathophysiology Experimental, Postgraduate Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, Brazil.
Graduate Program of Biomedical Science, Herminio Ometto Foundation, Araras, Brazil.
Ultrasound Med Biol. 2025 Jan;51(1):57-69. doi: 10.1016/j.ultrasmedbio.2024.09.007. Epub 2024 Oct 11.
To develop a treatment that enhances recovery from envenomation-induced lesions caused by Bothrops jararaca venom by using ultrasound in combination with gold nanoparticles (GNPs).
A total of 108 Swiss mice were arranged into nine groups. The animals underwent necrotic induction with 250 µg B. jararaca venom (BjV) and were treated with ultrasound (U) at 1 MHz frequency at an intensity of 0.8 W/cm² for 5 min, 30 mg/L GNPs, and anti-bothropic serum (AS) in the following combinations: saline solution (SS); BjV; BjV + AS; BjV + AS + U; BjV + GNPs + AS; BjV + GNPs + AS + U; BjV + GNPs; BjV + GNPs + U; and BjV + U. The necrotic area, histology, oxidative stress, oxidative damage, and anti-oxidant system were assessed to evaluate the effects of the treatments.
Treatments that included GNPs, U, and/or AS demonstrated reductions in necrotic area, increases in angiogenesis and fibroblast means, decreases in inflammatory infiltrates, and improvements in collagen synthesis. Additionally, there was an increase in oxidants and oxidant damage within the gastrocnemius muscle, along with an increase in anti-oxidants. Furthermore, systemic effects appear to have been achieved, improving the anti-oxidant system at the cardiovascular and renal levels.
The use of GNPs and U may be effective at treating lesions caused by B. jararaca snake venom.
开发一种治疗方法，通过超声联合金纳米粒子（GNPs）来增强被 Bothrops jararaca 毒液引起的损伤的恢复。
将 108 只瑞士小鼠分为 9 组。动物接受 250µg B. jararaca 毒液（BjV）的坏死诱导，并接受 1 MHz 频率、0.8 W/cm²强度的超声处理 5 分钟，同时使用 30 mg/L GNPs 和抗 Bothropic 血清（AS）进行以下组合治疗：生理盐水溶液（SS）；BjV；BjV + AS；BjV + AS + U；BjV + GNPs + AS；BjV + GNPs + AS + U；BjV + GNPs；BjV + GNPs + U；BjV + U。评估坏死面积、组织学、氧化应激、氧化损伤和抗氧化系统，以评估治疗效果。
包含 GNPs、U 和/或 AS 的治疗方法可减少坏死面积，增加血管生成和成纤维细胞平均值，减少炎症浸润，并改善胶原合成。此外，腓肠肌中的氧化剂和氧化剂损伤增加，抗氧化剂增加。此外，似乎已经达到了全身效应，改善了心血管和肾脏水平的抗氧化系统。
GNPs 和 U 的使用可能对治疗 B. jararaca 蛇毒引起的损伤有效。
