Borgarelli M B, Marfort R, Sabelli C A, Marcantoni M, Mateo M T
Cátedra de Microbiología y Parasitología, Facultad de Odontología, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1985;17(1):41-6.
The St. mutans strains studied were: HS6 "a"; BHT "b"; IB "c"; B13 "d"; LM7 "e"; OMZ175 "f"; OMZ65 "g"; SL1 "SL"; 6715 "d/g". Immune sera and antigen preparation as well as antigen-antibody reactions were performed using the above-mentioned strains. The immune sera were obtained following Knox technique, including formolated immunogen with and without adjuvant. Linzer method was followed for rabbit immunization. The antigen extract were prepared according to Rantz and Randall method. The antigen-antibody reaction were carried out by microimmunodiffusion (MID) Ouchterlony technique and by counterimmunoelectrophoresis (CIE) according to De Torres method. A good correlation was found in the antigen-antibody reactions for the serotypes studied. MID and CIE revealed some cross-reactions, as already described by many investigators. The results obtained are compatible with St. mutans antigenic instability. The eight serotypes from St. mutans could possibly be reduced to four related groups: Group 1: serotypes "a-d-g-SL"; Group 2: serotype "e-f"; Group 3: serotype "c" (uncertain position: it can be related with group 1 or 2); Group 4: serotype "b". CIE is a convenient technique to be incorporated for immunological determination.
HS6 “a”；BHT “b”；IB “c”；B13 “d”；LM7 “e”；OMZ175 “f”；OMZ65 “g”；SL1 “SL”；6715 “d/g”。使用上述菌株进行免疫血清和抗原制备以及抗原 - 抗体反应。免疫血清按照诺克斯技术获得，包括使用和不使用佐剂的福尔马林化免疫原。采用林泽方法对兔子进行免疫。抗原提取物根据兰茨和兰德尔方法制备。抗原 - 抗体反应通过微量免疫扩散（MID）奥克特洛尼技术以及根据德托雷斯方法的对流免疫电泳（CIE）进行。在所研究的血清型的抗原 - 抗体反应中发现了良好的相关性。如许多研究者所描述的，MID和CIE显示出一些交叉反应。所获得的结果与变形链球菌的抗原不稳定性相符。变形链球菌的八种血清型可能可归为四个相关组：第1组：血清型“a - d - g - SL”；第2组：血清型“e - f”；第3组：血清型“c”（位置不确定：它可能与第1组或第2组相关）；第4组：血清型“b”。CIE是一种便于纳入免疫测定的技术。
