• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

SH3和多个锚蛋白重复结构域2表达升高与胶质瘤预后改善相关。

Elevated SH3 and Multiple Ankyrin Repeat Domains 2 Expression Correlates With Improved Glioma Prognosis.

作者信息

Li Wenlin, Shi Haiping, He Jimin

机构信息

Department of Neurosurgery, Suining Central Hospital, Suining, Sichuan, China.

出版信息

Int J Genomics. 2024 Oct 4;2024:6565925. doi: 10.1155/2024/6565925. eCollection 2024.

DOI:10.1155/2024/6565925
PMID:39397895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11469935/
Abstract

This study investigates the prognostic significance of SH3 and multiple ankyrin repeat domains 2 (SHANK2) gene expression in glioma patients, using data from The Cancer Genome Atlas (TCGA), the Genotype-Tissue Expression (GTEx) project, and the Gene Expression Omnibus (GEO). Through comprehensive analysis, we found a significant association between higher SHANK2 expression and improved survival outcomes across various glioma subtypes. To further validate the clinical relevance of SHANK2, we conducted cellular experiments involving siRNA-mediated knockdown of SHANK2 in U87 and A172 glioma cell lines. Quantitative real-time PCR (qPCR) and Western blot analyses confirmed the successful knockdown of SHANK2, and subsequent MTT assays revealed that silencing SHANK2 significantly promoted glioma cell proliferation. These findings underscore the potential role of SHANK2 as a tumor suppressor in glioma. The study also introduces a multivariate prognostic model incorporating SHANK2, providing a novel perspective on glioma prognosis. While the retrospective nature of the study presents limitations, our results suggest that SHANK2 expression could serve as a valuable biomarker for glioma prognosis and inform future therapeutic strategies.

摘要

本研究利用来自癌症基因组图谱（TCGA）、基因型-组织表达（GTEx）项目和基因表达综合数据库（GEO）的数据，调查了SH3和多个锚蛋白重复结构域2（SHANK2）基因表达在胶质瘤患者中的预后意义。通过综合分析，我们发现在各种胶质瘤亚型中，较高的SHANK2表达与改善的生存结果之间存在显著关联。为了进一步验证SHANK2的临床相关性，我们进行了细胞实验，在U87和A172胶质瘤细胞系中采用小干扰RNA（siRNA）介导的SHANK2敲低。定量实时聚合酶链反应（qPCR）和蛋白质免疫印迹分析证实了SHANK2的成功敲低，随后的MTT实验表明，沉默SHANK2显著促进了胶质瘤细胞的增殖。这些发现强调了SHANK2作为胶质瘤肿瘤抑制因子的潜在作用。该研究还引入了一个包含SHANK2的多变量预后模型，为胶质瘤预后提供了新的视角。虽然该研究的回顾性性质存在局限性，但我们的结果表明，SHANK2表达可作为胶质瘤预后的有价值生物标志物，并为未来的治疗策略提供参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/ecae06b0ca81/IJG2024-6565925.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/efe0ba3044aa/IJG2024-6565925.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/b7159fa9a8f5/IJG2024-6565925.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/cce77b837015/IJG2024-6565925.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/a2aedd7b0d47/IJG2024-6565925.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/7064974fab94/IJG2024-6565925.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/ecae06b0ca81/IJG2024-6565925.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/efe0ba3044aa/IJG2024-6565925.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/b7159fa9a8f5/IJG2024-6565925.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/cce77b837015/IJG2024-6565925.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/a2aedd7b0d47/IJG2024-6565925.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/7064974fab94/IJG2024-6565925.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/11469935/ecae06b0ca81/IJG2024-6565925.006.jpg

相似文献

1
Elevated SH3 and Multiple Ankyrin Repeat Domains 2 Expression Correlates With Improved Glioma Prognosis.SH3和多个锚蛋白重复结构域2表达升高与胶质瘤预后改善相关。
Int J Genomics. 2024 Oct 4;2024:6565925. doi: 10.1155/2024/6565925. eCollection 2024.
2
UBR1 is a prognostic biomarker and therapeutic target associated with immune cell infiltration in gastric cancer.UBR1 是一种与胃癌免疫细胞浸润相关的预后生物标志物和治疗靶点。
Aging (Albany NY). 2024 Aug 23;16(16):12029-12049. doi: 10.18632/aging.206079.
3
Cuproptosis-related gene SLC31A1 expression correlates with the prognosis and tumor immune microenvironment in glioma.铜死亡相关基因 SLC31A1 的表达与胶质瘤的预后和肿瘤免疫微环境相关。
Funct Integr Genomics. 2023 Aug 23;23(3):279. doi: 10.1007/s10142-023-01210-0.
4
CBX3 promotes glioma U87 cell proliferation and predicts an unfavorable prognosis.CBX3 促进脑胶质瘤 U87 细胞增殖并预测不良预后。
J Neurooncol. 2019 Oct;145(1):35-48. doi: 10.1007/s11060-019-03286-w. Epub 2019 Sep 9.
5
[Effects of FPR2 gene silencing on the proliferation, migration and invasion of human glioma U87 cells].FPR2基因沉默对人胶质瘤U87细胞增殖、迁移及侵袭的影响
Zhonghua Zhong Liu Za Zhi. 2018 Sep 23;40(9):659-666. doi: 10.3760/cma.j.issn.0253-3766.2018.09.004.
6
Transmembrane and Ubiquitin-Like Domain-Containing 1 Promotes Glioma Growth and Indicates Unfavorable Prognosis.跨膜和含泛素样结构域蛋白1促进胶质瘤生长并提示预后不良。
Int J Genomics. 2023 Dec 19;2023:3318171. doi: 10.1155/2023/3318171. eCollection 2023.
7
Effect of the autism-associated lncRNA Shank2-AS on architecture and growth of neurons.自闭症相关长链非编码RNA Shank2-AS对神经元结构和生长的影响。
J Cell Biochem. 2019 Feb;120(2):1754-1762. doi: 10.1002/jcb.27471. Epub 2018 Aug 30.
8
Genetic Analysis Implicates Dysregulation of in Renal Cell Carcinoma Progression.遗传分析表明在肾细胞癌进展中存在的失调。
Int J Environ Res Public Health. 2022 Sep 30;19(19):12471. doi: 10.3390/ijerph191912471.
9
DNMT3A promotes glioma growth and malignancy via TNF-α/NF-κB signaling pathway.DNA甲基转移酶3A通过肿瘤坏死因子-α/核因子-κB信号通路促进胶质瘤生长和恶性进展。
Transl Cancer Res. 2024 Apr 30;13(4):1786-1806. doi: 10.21037/tcr-23-1943. Epub 2024 Apr 22.
10
High LYRM4-AS1 predicts poor prognosis in patients with glioma and correlates with immune infiltration.高表达 LYRM4-AS1 预测胶质瘤患者预后不良，并与免疫浸润相关。
PeerJ. 2023 Oct 3;11:e16104. doi: 10.7717/peerj.16104. eCollection 2023.

本文引用的文献

1
Oncolytic viral therapy for gliomas: Advances in the mechanisms and approaches to delivery.溶瘤病毒疗法治疗脑胶质瘤：作用机制及给药途径的研究进展。
Virology. 2024 May;593:110033. doi: 10.1016/j.virol.2024.110033. Epub 2024 Feb 20.
2
Efficient diagnosis of IDH-mutant gliomas: 1p/19qNET assesses 1p/19q codeletion status using weakly-supervised learning.异柠檬酸脱氢酶（IDH）突变型胶质瘤的高效诊断：1p/19qNET利用弱监督学习评估1p/19q共缺失状态。
NPJ Precis Oncol. 2023 Sep 16;7(1):94. doi: 10.1038/s41698-023-00450-4.
3
Structural deficits in key domains of Shank2 lead to alterations in postsynaptic nanoclusters and to a neurodevelopmental disorder in humans.
Shank2 关键结构域的结构性缺陷导致突触后纳米簇的改变，并导致人类神经发育障碍。
Mol Psychiatry. 2024 Jun;29(6):1683-1697. doi: 10.1038/s41380-022-01882-3. Epub 2022 Nov 30.
4
Genetic Analysis Implicates Dysregulation of in Renal Cell Carcinoma Progression.遗传分析表明在肾细胞癌进展中存在的失调。
Int J Environ Res Public Health. 2022 Sep 30;19(19):12471. doi: 10.3390/ijerph191912471.
5
Glioma progression is shaped by genetic evolution and microenvironment interactions.胶质瘤的进展是由遗传进化和微环境相互作用决定的。
Cell. 2022 Jun 9;185(12):2184-2199.e16. doi: 10.1016/j.cell.2022.04.038. Epub 2022 May 31.
6
Epidemiology of Glioblastoma Multiforme-Literature Review.多形性胶质母细胞瘤的流行病学——文献综述
Cancers (Basel). 2022 May 13;14(10):2412. doi: 10.3390/cancers14102412.
7
Expression of SH3 and Multiple Ankyrin Repeat Domains Protein 3 in Mouse Retina.SH3和多个锚蛋白重复结构域蛋白3在小鼠视网膜中的表达
Front Cell Neurosci. 2022 Mar 25;16:795668. doi: 10.3389/fncel.2022.795668. eCollection 2022.
8
Diffuse Glioma Heterogeneity and Its Therapeutic Implications.弥漫性胶质瘤的异质性及其治疗意义。
Cancer Discov. 2021 Mar;11(3):575-590. doi: 10.1158/2159-8290.CD-20-1474. Epub 2021 Feb 8.
9
Role of PDZ-binding motif from West Nile virus NS5 protein on viral replication.西尼罗河病毒 NS5 蛋白 PDZ 结合基序在病毒复制中的作用。
Sci Rep. 2021 Feb 5;11(1):3266. doi: 10.1038/s41598-021-82751-x.
10
SHANK2 mutations impair apoptosis, proliferation and neurite outgrowth during early neuronal differentiation in SH-SY5Y cells.SHANK2 突变会损害 SH-SY5Y 细胞早期神经元分化过程中的细胞凋亡、增殖和神经突生长。
Sci Rep. 2021 Jan 22;11(1):2128. doi: 10.1038/s41598-021-81241-4.