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该基因对[具体病原体名称]致病性及免疫功能的影响。 （你提供的原文中“Effect of the gene on the pathogenicity of and immune function.”这里有部分内容缺失，我按照正常逻辑补充了“[具体病原体名称]”，若实际不是这样，请根据正确内容调整）

Effect of the gene on the pathogenicity of and immune function .

作者信息

Li Mengyan, Wang Hua, Zhao Huihai, Jiang Mengyu, Cui Mengge, Jia Keran, Lei Daxin, Wang Fukun

机构信息

Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, China.

出版信息

Heliyon. 2024 Sep 25;10(19):e38219. doi: 10.1016/j.heliyon.2024.e38219. eCollection 2024 Oct 15.

DOI:10.1016/j.heliyon.2024.e38219
PMID:39397919
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11467569/
Abstract

OBJECTIVES

a ubiquitous membrane protein in fungi, plays a pivotal role in various physiological processes, such as osmotic stress, oxidative stress, temperature response, and virulence regulation across different fungal species. This study aimed to investigate the effect of the gene on the pathogenicity of and its immune function .

MATERIALS AND METHODS

Ninety-nine clinical strains from various infection sites were collected to investigate the expression levels of the gene compared to its levels in the standard strain (SC5314). -knockout strains () were constructed to investigate the impact of the gene deletion on the biofilm formation, adhesion, and flocculation abilities of . A mouse model of systemic infection was established to evaluate the impact of deletion on survival, organ pathology, and immune cell function, as assessed by flow cytometry.

RESULTS

The expression level of the gene was found to be higher in clinical strains derived from sterile fluids, sputum, and secretions compared to that in the standard strains. Deletion of the gene diminished the biofilm-formation capacity of , leading to a sparse structure and reduced thickness, as well as diminished adhesion and flocculation abilities. Deletion of the gene prolonged mouse survival; decreased the fungal load in the liver, kidney, and spleen; and reduced inflammatory cell infiltration into the kidney. In the spleens of mice injected with the strain, a decrease was observed in the percentage of M1-type macrophages and an increase in M2-type macrophages, resulting in a decreased M1/M2 macrophage ratio. Additionally, an increase was observed in the number of Th1 cells and a decrease in the number of Th2 and Th17 cells, leading to an increased Th1/Th2 ratio.

CONCLUSION

The gene significantly contributes to the pathogenesis of by influencing its biological behaviour and immune response .

摘要

目的

一种在真菌中普遍存在的膜蛋白，在各种生理过程中起关键作用，如渗透胁迫、氧化应激、温度反应以及不同真菌物种间的毒力调节。本研究旨在探究该基因对[具体真菌名称]致病性及其免疫功能的影响。

材料与方法

收集来自不同感染部位的99株临床菌株，以研究该基因与标准菌株（SC5314）相比的表达水平。构建该基因敲除菌株（[具体敲除菌株名称]），以研究基因缺失对[具体真菌名称]生物膜形成、黏附及絮凝能力的影响。建立系统性感染小鼠模型，通过流式细胞术评估该基因缺失对小鼠生存、器官病理学及免疫细胞功能的影响。

结果

发现来自无菌体液、痰液和分泌物的临床菌株中该基因的表达水平高于标准菌株。该基因的缺失降低了[具体真菌名称]的生物膜形成能力，导致结构稀疏、厚度减小，同时黏附及絮凝能力下降。该基因的缺失延长了小鼠的生存期；降低了肝脏、肾脏和脾脏中的真菌载量；减少了肾脏中的炎性细胞浸润。在注射了[具体敲除菌株名称]的小鼠脾脏中，观察到M1型巨噬细胞百分比降低，M2型巨噬细胞增加，导致M1/M2巨噬细胞比值降低。此外，Th1细胞数量增加，Th2和Th17细胞数量减少，导致Th1/Th2比值升高。

结论

该基因通过影响[具体真菌名称]的生物学行为和免疫反应，对其致病性有显著贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/7aaabd6d095b/mmcfigs10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/50233910bc22/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/12db3d91e314/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/e0c56db453c3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/1265f7b6f32b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/b54135d46e10/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/41d5c58ac42c/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/92a78b820f36/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/e97b3cc7fa92/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/c947ae72a53d/mmcfigs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/2f21c06d1877/mmcfigs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/137d00a5932c/mmcfigs4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/b3bf59e71d7d/mmcfigs5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/4b2bf804d5bb/mmcfigs6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/92409168e958/mmcfigs7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/1320ade96664/mmcfigs8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/911c4d25fdd0/mmcfigs9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/7aaabd6d095b/mmcfigs10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/50233910bc22/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/12db3d91e314/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/e0c56db453c3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/1265f7b6f32b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/b54135d46e10/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/41d5c58ac42c/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/92a78b820f36/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/e97b3cc7fa92/mmcfigs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/c947ae72a53d/mmcfigs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/2f21c06d1877/mmcfigs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/137d00a5932c/mmcfigs4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/b3bf59e71d7d/mmcfigs5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/4b2bf804d5bb/mmcfigs6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/92409168e958/mmcfigs7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/1320ade96664/mmcfigs8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/911c4d25fdd0/mmcfigs9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddba/11467569/7aaabd6d095b/mmcfigs10.jpg

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