ficin 对白色念珠菌生物膜形成和已形成生物膜的抑制作用。
Inhibitory effect of ficin on Candida albicans biofilm formation and pre-formed biofilms.
机构信息
School and Hospital of Stomatology, Wenzhou Medical University, 325027, Wenzhou, People's Republic of China.
Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, People's Republic of China.
出版信息
BMC Oral Health. 2022 Aug 13;22(1):350. doi: 10.1186/s12903-022-02384-y.
BACKGROUND
To investigate the effect of ficin, a type of proteases, on Candida albicans (C. albicans) biofilm, including forming and pre-formed biofilms.
METHODS
Crystal violet tests together with colony forming unit (CFU) counts were used to detect fungal biofilm biomass. Live/dead staining of biofilms observed by confocal laser scanning microscopy was used to monitor fungal activity. Finally, gene expression of C. albicans within biofilms was assessed by qRT-PCR.
RESULTS
According to our results, biofilm biomass was dramatically reduced by ficin in both biofilm formation and pre-formed biofilms, as revealed by the crystal violet assay and CFU count (p < 0.05). Fungal activity in biofilm formation and pre-formed biofilms was not significantly influenced by ficin according to live/dead staining. Fungal polymorphism and biofilm associated gene expression were influenced by ficin, especially in groups with prominent antibiofilm effects.
CONCLUSIONS
In summary, ficin effectively inhibited C. albicans biofilm formation and detached its preformed biofilm, and it might be used to treat C. albicans biofilm associated problems.
背景
研究ficin （一种蛋白酶）对白色念珠菌（C. albicans）生物膜的影响，包括形成和预形成的生物膜。
方法
结晶紫试验和集落形成单位（CFU）计数用于检测真菌生物膜生物量。通过共聚焦激光扫描显微镜观察活/死染色来监测真菌活性。最后，通过 qRT-PCR 评估生物膜内 C. albicans 的基因表达。
结果
根据我们的结果，ficin 显著减少了生物膜生物量，无论是在生物膜形成还是预形成的生物膜中，这通过结晶紫测定和 CFU 计数（p < 0.05）得到证实。根据活/死染色，ficin 对生物膜形成和预形成生物膜中的真菌活性没有显著影响。真菌多态性和生物膜相关基因表达受 ficin 影响，尤其是在具有明显抗生物膜作用的组中。
结论
总之，ficin 能有效抑制 C. albicans 生物膜的形成并使其预形成的生物膜脱落，可用于治疗与 C. albicans 生物膜相关的问题。
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