Dibo Nouhoum, Zhou Zhijun, Liu Xianshu, Li Zhuolin, Zhong Shukun, Liu Yan, Duan Juan, Xia Meng, Ma Zhenrong, Wu Xiang, Huang Shuaiqin
Department of Medical Parasitology, Xiangya School of Basic Medicine, Central South University, Changsha, 410013, Hunan Province, China.
Department of Laboratory Animals, Hunan Key Laboratory of Animal Models for Human Diseases, Central South University, Changsha, 410013, Hunan, China.
Heliyon. 2024 Sep 26;10(19):e38067. doi: 10.1016/j.heliyon.2024.e38067. eCollection 2024 Oct 15.
is known as a non-susceptible animal host of . A better understanding of this animal immune defense mechanism during the early stage of infection may offer an alternative route for vaccine development or therapy. Here, we analyzed the whole blood transcriptome of using next-generation sequencing (NGS) to identify immune genes of biological relevance that might be involved in the mechanism of its resistance. The blood samples were collected from uninfected animals (control group) and infected animals at different time points (3, 7, 10 and 14 days post-infection). We identified 5310 sequences as unigenes and successfully annotated 4636 of them. The immune response was more intense at 10 dpi. The upregulated genes at this time point were mainly activated in the TNF and NF-kappa B signaling pathways, Th1, Th2and Th17 cell differentiation as well as cytokine-cytokine receptor interaction. Based on the differentially expressed genes analysis, we report that the , , , , , , , , , , and could play important roles in the mechanism of resistance.
被认为是……的非易感动物宿主。在感染早期更好地了解这种动物的免疫防御机制可能为疫苗开发或治疗提供另一条途径。在此，我们使用下一代测序（NGS）分析了……的全血转录组，以鉴定可能参与其抗性机制的具有生物学相关性的免疫基因。血液样本从未感染动物（对照组）和感染后不同时间点（感染后3、7、10和14天）的感染动物中采集。我们将5310个序列鉴定为单基因，并成功注释了其中4636个。在感染后10天免疫反应更强。此时上调的基因主要在TNF和NF-κB信号通路、Th1、Th2和Th17细胞分化以及细胞因子-细胞因子受体相互作用中被激活。基于差异表达基因分析，我们报告……在……抗性机制中可能发挥重要作用。
