Department of Plant Sciences, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai 200240, China.
Plant Sci. 2025 Jan;350:112279. doi: 10.1016/j.plantsci.2024.112279. Epub 2024 Oct 12.
SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes are plant-specific transcription factors essential for plant growth, development, and stress responses. Their roles in sweet cherry are not well understood. In this study, we identified and isolated 16 SPL genes from the sweet cherry genome, categorizing them into 5 subfamilies, with 12 PavSPLs predicted as miR156 targets. Promoter regions of PavSPLs contain cis-elements associated with light, stress, and phytohormone responses, indicating their role in biological processes and abiotic stress responses. Seasonal expression analysis showed that PavSPL regulates sweet cherry recovery after dormancy. Gibberellin (GA) treatment reduced PavSPL expression, indicating its role in GA-mediated processes. PavSPL14 overexpression in Arabidopsis thaliana resulted in earlier flowering and increased plant height and growth. Yeast two-hybrid assays showed an interaction between PavSPL14 and DELLA protein PavDWARF8, suggesting PavSPL14 and PavDWARF8 co-regulate growth and development. These findings lay the groundwork for further research on PavSPL function in sweet cherry.
SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) 基因是植物特有的转录因子，对植物的生长、发育和应激反应至关重要。它们在甜樱桃中的作用尚未得到很好的理解。在这项研究中，我们从甜樱桃基因组中鉴定并分离出 16 个 SPL 基因，将它们分为 5 个亚家族，其中 12 个 PavSPLs 被预测为 miR156 的靶标。PavSPLs 的启动子区域包含与光、应激和植物激素反应相关的顺式元件，表明它们在生物过程和非生物应激反应中的作用。季节性表达分析表明，PavSPL 调节甜樱桃休眠后的恢复。赤霉素（GA）处理降低了 PavSPL 的表达，表明其在 GA 介导的过程中的作用。在拟南芥中过表达 PavSPL14 导致更早开花和增加株高和生长。酵母双杂交试验表明 PavSPL14 与 DELLA 蛋白 PavDWARF8 之间存在相互作用，表明 PavSPL14 和 PavDWARF8 共同调节生长和发育。这些发现为进一步研究 PavSPL 在甜樱桃中的功能奠定了基础。
