The Second Affiliated Hospital, Shandong First Medical University and Shandong Academy of Medical Sciences, Taian, Shandong 271000, China.
Binhai County People's Hospital, Yancheng, Jiangsu 224500, China.
ACS Sens. 2024 Oct 25;9(10):5058-5068. doi: 10.1021/acssensors.4c00077. Epub 2024 Oct 14.
Spinal cord injury (SCI) is a serious neurological injury that is currently extremely difficult to cure clinically. SCI involves numerous pathophysiological processes, and microRNAs (miRNAs) play an important role in these processes. Meanwhile, miRNAs have received a lot of attention for their role in other diseases as well. Therefore, the detection of disease-related miRNAs is important for the study of disease development, treatment, and prognosis. With the rapid development of molecular biology, the traditional detection methods of miRNA can no longer meet the needs of experiments. Electrochemical detection methods are widely used because of their excellent detection performance. Here, we designed an electrochemical sensor prepared using borosilicate glass microneedle electrodes for real-time monitoring of miR-21-5p expression in vivo after SCI. The sensor showed a good linear relationship between the oxidation peak current value and the concentration of miR-21-5p in the concentration range 0-2 fM ( = 12.025 + 90.396, = 0.98). The limit of detection (LOD) of the sensor was 0.3667 fM. The experimental results showed that the borosilicate glass microneedle electrochemical sensor achieved fast, accurate, highly sensitive, highly specific, highly stable, and reproducible monitoring of miR-21-5p. More importantly, the electrochemical sensor has a better clinical translation prospect, which is important for the research of clinical diseases.
脊髓损伤（SCI）是一种严重的神经系统损伤，目前临床上极难治愈。SCI 涉及多个病理生理过程，miRNAs（miRNA）在这些过程中发挥着重要作用。同时，miRNA 在其他疾病中的作用也引起了广泛关注。因此，检测与疾病相关的 miRNA 对于研究疾病的发展、治疗和预后非常重要。随着分子生物学的飞速发展，传统的 miRNA 检测方法已经不能满足实验的需要。电化学检测方法因其优异的检测性能而得到广泛应用。在这里，我们设计了一种使用硼硅酸盐玻璃微针电极制备的电化学传感器，用于实时监测 SCI 后体内 miR-21-5p 的表达。该传感器在 0-2 fM（ = 12.025 + 90.396， = 0.98）浓度范围内表现出良好的氧化峰电流值与 miR-21-5p 浓度之间的线性关系。传感器的检测限（LOD）为 0.3667 fM。实验结果表明，硼硅酸盐玻璃微针电化学传感器实现了对 miR-21-5p 的快速、准确、高灵敏度、高特异性、高稳定性和可重复性监测。更重要的是，电化学传感器具有更好的临床转化前景，对临床疾病的研究具有重要意义。
