State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha, China.
Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.
Nat Plants. 2024 Nov;10(11):1761-1774. doi: 10.1038/s41477-024-01823-8. Epub 2024 Oct 14.
Plant roots exhibit localized immunity (LI) mainly in the transition zone (TZ) and elongation zone (EZ). Plasma membrane-localized receptor-like kinases (RLKs) can mediate the plant's response to rhizosphere bacteria. However, how RLKs are involved in triggering LI in roots remains unclear. Here we identified dual actions for the RLK FERONIA (FER) in the LI response of Arabidopsis (Arabidopsis thaliana). The FER cytoplasmic domain is cleaved and translocated to the nucleus (FERN) to activate LI in the TZ and EZ in response to colonization by beneficial and pathogenic bacteria. In the absence or cessation of bacterial infection, full-length FER is plasma membrane-localized to maintain growth. Upon colonization and invasion by a high titre of bacteria, mature RAPID ALKALINIZATION FACTOR23 peptide accumulates and activates the matrix metalloproteinase At2-MMP, which triggers FER cytoplasmic domain cleavage specifically in the TZ and EZ to activate LI. This work demonstrates that two molecular forms of a single RLK balance growth and immunity via LI activation in Arabidopsis roots.
植物根系在过渡区（TZ）和伸长区（EZ）表现出局部免疫（LI）。质膜定位的受体样激酶（RLKs）可以介导植物对根际细菌的反应。然而，RLKs 如何参与触发根系中的 LI 尚不清楚。在这里，我们确定了拟南芥（Arabidopsis thaliana）LI 反应中 RLK FERONIA（FER）的双重作用。FER 胞质结构域被切割并转移到核内（FERN），以响应有益和病原细菌的定植激活 TZ 和 EZ 中的 LI。在没有或停止细菌感染的情况下，全长 FER 定位于质膜以维持生长。在高浓度细菌的定植和入侵下，成熟的 RAPID ALKALINIZATION FACTOR23 肽积累并激活基质金属蛋白酶 At2-MMP，该酶特异性地在 TZ 和 EZ 中触发 FER 胞质结构域切割，以激活 LI。这项工作表明，单一 RLK 的两种分子形式通过在拟南芥根系中激活 LI 来平衡生长和免疫。
