Skalidis G, Trifilieff E, Luu B
J Neurochem. 1986 Jan;46(1):297-9. doi: 10.1111/j.1471-4159.1986.tb12962.x.
Brain DM-20 proteolipid was previously shown to be structurally different from the myelin major proteolipid (MMPL). In an attempt to set up a large-scale purification of DM-20, we studied extraction of brain proteolipids with mixtures of methylene chloride containing up to 80% methanol. The CH2Cl2-CH3OH (3:7, vol/vol) mixture is highly selective for the extraction of bovine brain DM-20 compared to MMPL. Purified DM-20 was obtained after chromatography of the extract on methylated Sephadex.
脑DM - 20蛋白脂质先前已被证明在结构上不同于髓鞘主要蛋白脂质（MMPL）。为了建立大规模纯化DM - 20的方法，我们研究了用含高达80%甲醇的二氯甲烷混合物提取脑蛋白脂质。与MMPL相比，CH2Cl2 - CH3OH（3:7，体积/体积）混合物对牛脑DM - 20的提取具有高度选择性。提取物经甲基化葡聚糖凝胶柱层析后得到纯化的DM - 20。
