Integrated Program in Neuroscience, McGill University, Montreal, QC H3A 1A1, Canada.
Clinical and Translational Sciences Lab, Douglas Research Centre, Montreal, QC H4H 1R3, Canada.
Cells. 2024 Sep 29;13(19):1628. doi: 10.3390/cells13191628.
Astrocytic reactivity in substance use disorders (SUDs) has been extensively studied, yet the molecular effect of delta-9-tetrahydrocannabinol (∆9-THC, the main psychoactive compound in cannabis) on glial cells, especially astrocytes, remains poorly understood. Exploring ∆9-THC's impact on astrocytic markers can provide insight into its effects on brain functions such as homeostasis, synaptic transmission, and response to neuronal injury. This systematic review synthesizes findings from studies investigating ∆9-THC's impact on astrocytic markers.
A systematic review was conducted using EMBASE, Medline, and PsychoInfo via the OvidSP platform. Studies reporting astrocytic markers following ∆9-THC exposure in animals and humans were included. Data were extracted from twelve eligible full-text articles, and the risk of bias was assessed using the Systematic Review Center for Laboratory Animal Experimentation.
This research identified several astrocytic markers, including glial fibrillary acidic protein (GFAP), nestin, and glutamate-aspartate transporter (GLAST). Both GFAP and nestin expressions increased in adulthood following adolescence and adult ∆9-THC exposure. An increase in GLAST expression was also noted during early development after ∆9-THC exposure.
This review indicates varying levels of astrocytic reactivity to ∆9-THC across different developmental stages, including adolescence and adulthood. ∆9-THC appears to impact maturation, particularly during early developmental stages, and exhibits sex-dependent effects.
在物质使用障碍（SUD）中，星形胶质细胞反应已经得到了广泛的研究，但 δ-9-四氢大麻酚（∆9-THC，大麻中的主要精神活性化合物）对神经胶质细胞，特别是星形胶质细胞的分子影响仍知之甚少。探索 ∆9-THC 对星形胶质细胞标志物的影响可以深入了解其对大脑功能的影响，如内稳态、突触传递和对神经元损伤的反应。本系统综述综合了研究 ∆9-THC 对星形胶质细胞标志物影响的研究结果。
通过 OvidSP 平台在 EMBASE、Medline 和 PsychoInfo 上进行系统综述。纳入报告动物和人类暴露于 ∆9-THC 后星形胶质细胞标志物的研究。从 12 篇合格的全文文章中提取数据，并使用系统评价中心进行实验室动物实验评估偏倚风险。
这项研究确定了几种星形胶质细胞标志物，包括胶质纤维酸性蛋白（GFAP）、巢蛋白和谷氨酸-天冬氨酸转运蛋白（GLAST）。青春期和成年期暴露于 ∆9-THC 后，GFAP 和巢蛋白的表达均在成年期增加。暴露于 ∆9-THC 后早期发育期间也观察到 GLAST 表达增加。
本综述表明，在不同的发育阶段，包括青春期和成年期，星形胶质细胞对 ∆9-THC 的反应程度不同。∆9-THC 似乎对成熟有影响，特别是在早期发育阶段，并且表现出性别依赖性效应。
