Yu Wenbang, Chen Xiaofen, Li Xinjie, Wei Zihan, Tang Jing, Zhang Min
Jinhua University of Vocational Technology, Jinhua, 321017, China.
School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.
Talanta. 2025 Jan 1;282:127039. doi: 10.1016/j.talanta.2024.127039. Epub 2024 Oct 11.
In this study, we amplified the capabilities of laser-induced graphene (LIG) by developing a multi-doped LIG extended-gated field-effect transistor (EG-FET) sensor. This sensor integrates a multi-doped LIG EG electrode array as a disposable sensing component with a standard MOSFET for reusable transduction. The multi-doped LIG was synthesized using a dual-approach: initially, by using a MnCl-doped polyimide (MnCl-PI) film through precursor compounding, and subsequently, by employing a CO laser to respectively in situ generate MnO nanoparticles and gold nanoparticles (Au NPs) via direct laser conversion. By incorporating the resultant multi-doped LIG (Au NPs/MnO/LIG) as the EG electrode, we boosted its electrical efficiency and provided ideal sites for the papain immobilization. This facilitated the selective binding of protein complexes with cystatin C (Cys C), allowing for precise measurement. Notably, the sensor exhibited a robust linear correlation across a concentration range from 50 ag/μL to 0.25 ng/μL and achieved a detection limit of 50 ag/μL. These advancements not only address traditional limitations of LIG applications but also highlight the potential of LIG-based EG-FET portable devices for accurate and early screening of chronic kidney disease (CKD).
在本研究中，我们通过开发一种多掺杂激光诱导石墨烯（LIG）扩展栅场效应晶体管（EG-FET）传感器，增强了LIG的性能。该传感器将多掺杂LIG EG电极阵列作为一次性传感组件与标准MOSFET集成，用于可重复使用的信号转换。多掺杂LIG采用双方法合成：首先，通过前体复合使用掺杂MnCl的聚酰亚胺（MnCl-PI）薄膜，随后，通过使用CO激光分别通过直接激光转换原位生成MnO纳米颗粒和金纳米颗粒（Au NPs）。通过将所得的多掺杂LIG（Au NPs/MnO/LIG）用作EG电极，我们提高了其电效率，并为木瓜蛋白酶固定提供了理想位点。这促进了蛋白质复合物与胱抑素C（Cys C）的选择性结合，从而实现精确测量。值得注意的是，该传感器在50 ag/μL至0.25 ng/μL的浓度范围内表现出稳健的线性相关性，检测限达到50 ag/μL。这些进展不仅解决了LIG应用的传统局限性，还突出了基于LIG的EG-FET便携式设备在慢性肾脏病（CKD）准确早期筛查方面的潜力。
