Hu Yanru, Chen Haolan, Li Huihui, Wang Yue, Zheng Xiukun, Liu Qing, Wen Qing, Shen Xiaoye, Wang Fengqin, Qi Yuancheng, Shen Jinwen
Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Henan, Zhengzhou 450002, People's Republic of China.
College of Food Science and Technology, Henan Agricultural University, Henan, Zhengzhou 450002, People's Republic of China.
J Agric Food Chem. 2024 Nov 13;72(45):25054-25065. doi: 10.1021/acs.jafc.4c04199. Epub 2024 Nov 4.
High-temperature or heat stress (HS) represents a significant environmental challenge that adversely affects crop growth and poses a substantial threat to agricultural production. , recognized as the second most widely cultivated edible fungus worldwide, is particularly susceptible to the detrimental effects of HS. Enhancing the HS resistance of is crucial for increasing its yield. In a prior investigation, we discovered that salicylic acid (SA) enhanced the resistance of mycelia to HS through a metabolic rearrangement. The present study further investigated the effects of SA on under HS. Cultivation experiments revealed that exogenous SA improved the mycelium recovery growth rate, yield, and fruiting body quality after HS. Further experiments revealed that exogenous SA mitigated the damage to the MAPK-Slt2 signal produced by HS while maintaining cell wall integrity. Furthermore, we hypothesized that the phenylalanine ammonia-lyase pathway might serve as a source for SA. In this context, we identified two salicylic hydroxylases, Po1102164 and Po1104438. Both HS and exogenous SA were found to elevate intracellular SA levels, thereby enhancing the resistance of to HS.
高温或热应激（HS）是一种严重的环境挑战，它会对作物生长产生不利影响，并对农业生产构成重大威胁。香菇，被认为是世界上第二大广泛栽培的食用菌，特别容易受到 HS 的不利影响。提高香菇对 HS 的抗性对于提高其产量至关重要。在之前的研究中，我们发现水杨酸（SA）通过代谢重排增强了香菇菌丝体对 HS 的抗性。本研究进一步研究了 SA 对 HS 下香菇的影响。培养实验表明，外源 SA 可以提高 HS 后菌丝体的恢复生长速度、产量和子实体质量。进一步的实验表明，外源 SA 减轻了 HS 引起的 MAPK-Slt2 信号损伤，同时保持了细胞壁的完整性。此外，我们假设苯丙氨酸解氨酶途径可能是 SA 的来源。在这种情况下，我们鉴定了两个水杨酸羟化酶，Po1102164 和 Po1104438。HS 和外源 SA 都被发现可以提高细胞内 SA 水平，从而增强香菇对 HS 的抗性。
