Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Saarland University, 66123, Saarbrücken, Germany.
Department of Pharmacy, Saarland University, 66123, Saarbrücken, Germany.
NPJ Biofilms Microbiomes. 2024 Oct 15;10(1):106. doi: 10.1038/s41522-024-00569-7.
Bacterial biofilms represent a prominent biological barrier against physical and chemical attacks. Disturbing the anaerobic microenvironment within biofilms by co-delivery of oxygen appears as a promising strategy to enhance the activity of an antibiotic. Here, we report the effect of oxygen-producing calcium peroxide nanoparticles (CaO NP) in combination with tobramycin sulfate (Tob). On Pseudomonas aeruginosa PAO1 biofilms in vitro, the additive effect of CaO NP towards Tob activity enhanced biofilm eradication by 2 log compared to Tob alone. For natural biofilms grown in the oral cavity of human volunteers in situ, treatment by CaO NP alone slightly increased the fraction of dead bacteria from 44% in various controls, including Tob alone, to 57%. However, the combination of CaO NP with Tob further increased the fraction of dead bacteria to 69%. These data confirm the intrinsic antimicrobial and antibiotic-potentiating effect of CaO NP also in a clinically relevant setting.
细菌生物膜是一种重要的生物学屏障，可以抵御物理和化学攻击。通过共递送氧气来破坏生物膜内的厌氧微环境，似乎是增强抗生素活性的一种有前途的策略。在这里，我们报告了产氧过氧化钙纳米颗粒（CaO NP）与硫酸妥布霉素（Tob）联合使用对铜绿假单胞菌 PAO1 生物膜的影响。在体外，与 Tob 单独使用相比，CaO NP 对 Tob 活性的增效作用使生物膜清除率增加了 2 个对数级。对于在人体志愿者口腔中原位生长的天然生物膜，CaO NP 单独处理将各种对照（包括 Tob 单独处理）中活菌的比例从 44%略有增加到 57%。然而，CaO NP 与 Tob 的联合使用进一步将死亡细菌的比例增加到 69%。这些数据证实了 CaO NP 具有内在的抗菌和增强抗生素作用，这在临床相关环境中也是如此。
