Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Int J Mol Sci. 2024 Sep 24;25(19):10276. doi: 10.3390/ijms251910276.
Phenylalanine ammonia-lyase (PAL) acts as the rate-limiting enzyme for anthocyanin biosynthesis through the phenylpropanoid pathway, a crucial component of plant secondary metabolism. The gene family plays a crucial role in plants' defense and stress responses, but its in silico identification and expression analyses in under different abiotic stresses remain unexplored. In this study, nine , seven , four , and seventeen genes were obtained from the genomes of , , , and , respectively. Segmental duplication and purifying selection are the causes of the gene's amplification and evolution. The genes with comparable intron-exon architectures and motifs were grouped together in the same clade. Three categories comprised the -regulatory elements: abiotic stressors, phytohormones, and light. According to the results of the qRT-PCR experiments, the majority of the genes were expressed highly under MeJA, a low temperature, and a high temperature, and they were downregulated under ABA. Under white light (100 µmol m s) with 50, 100, or 150 µmol m s far-red (FR), only a small number of the genes were expressed at 50 and 100 µmol m s FR, while the majority of the genes were slightly elevated at 150 µmol m s FR. This work offers a theoretical foundation for molecular breeding research to investigate the role of genes and their role in anthocyanin biosynthesis.
苯丙氨酸解氨酶（PAL）作为苯丙烷途径中花色苷生物合成的限速酶，是植物次生代谢的重要组成部分。该基因家族在植物的防御和应激反应中起着关键作用，但在不同非生物胁迫下其在计算机上的鉴定和表达分析仍未被探索。在这项研究中，从、、和的基因组中分别获得了九个、七个、四个和十七个基因。片段重复和纯化选择是基因扩增和进化的原因。具有可比内含子-外显子结构和基序的基因被分组到同一分支中。-调控元件包括非生物胁迫、植物激素和光三个类别。根据 qRT-PCR 实验的结果，大多数基因在 MeJA、低温和高温下表达水平较高，而在 ABA 下表达水平较低。在白光（100 µmol m s）下用 50、100 或 150 µmol m s 远红光（FR）处理时，只有少数基因在 50 和 100 µmol m s FR 下表达，而大多数基因在 150 µmol m s FR 下略有升高。这项工作为分子育种研究提供了理论基础，以研究基因的作用及其在花色苷生物合成中的作用。
