Department of Sleep Medicine and Metabolic Disorders, Medical University of Lodz, Lodz, Poland.
Department of Biochemistry, Medical University of Lodz, Lodz, Poland.
Transl Psychiatry. 2024 Mar 22;14(1):158. doi: 10.1038/s41398-024-02871-6.
Sleep deprivation (DS) is the forced elimination of sleep. While brain-derived neurotrophic factor (BDNF) has been extensively studied in the context of in mood changes following DS, the role of other neurotrophins remains elusive. This study explores the impact of DS on BDNF, glial cell line-derived neurotrophic factor (GDNF), neurotrophin-3 (NT3), and neurotrophin-4 (NT4) at mRNA and protein level, considering their potential links to mood disturbances. The study involved 81 participants subjected to polysomnography (PSG) and DS. Blood samples, mood assessments, and actigraphy data were collected twice, after PSG and DS. NT mRNA expression and serum protein concentrations of BDNF, GDNF, NT3, and NT4 were measured. Participants were divided into Responders and Non-Responders based on mood improvement after DS. DS reduced BDNF mRNA expression in all participants, with no change in serum BDNF protein. GDNF protein decreased in Non-Responders, while Responders exhibited reduced GDNF mRNA. NT3 protein increased in both groups, while NT3 mRNA decreased in Respondents. NT4 protein rose universally post-DS, but NT4 mRNA remained unchanged. Physical activity (PA) negatively correlated with mRNA expression of BDNF, GDNF, and NT3 post-DS. The study's short DS duration and exclusion of immature NT forms limit comprehensive insights. GDNF, together with NT3, might play an important role in mood response to DS. PA during DS seems to impair the mRNA expression of NTs in leukocytes. Future studies on the subject of sleep deprivation might consider investigating the relationship between BDNF and NT4 in the context of their apparent redundancy.
睡眠剥夺（DS）是强制消除睡眠。虽然脑源性神经营养因子（BDNF）在 DS 后情绪变化的背景下已经得到了广泛研究，但其他神经营养因子的作用仍然难以捉摸。本研究探讨了 DS 对 BDNF、胶质细胞源性神经营养因子（GDNF）、神经营养因子-3（NT3）和神经营养因子-4（NT4）在 mRNA 和蛋白水平上的影响，考虑到它们与情绪障碍的潜在联系。该研究涉及 81 名接受多导睡眠图（PSG）和 DS 的参与者。在 PSG 和 DS 后两次采集血液样本、情绪评估和活动数据。测量了 NT mRNA 表达和 BDNF、GDNF、NT3 和 NT4 的血清蛋白浓度。根据 DS 后情绪改善情况，将参与者分为反应者和非反应者。DS 降低了所有参与者的 BDNF mRNA 表达，而血清 BDNF 蛋白没有变化。GDNF 蛋白在非反应者中减少，而反应者表现出 GDNF mRNA 减少。两组 NT3 蛋白均增加，而反应者 NT3 mRNA 减少。DS 后 NT4 蛋白普遍升高，但 NT4 mRNA 不变。DS 后，PA 与 BDNF、GDNF 和 NT3 的 mRNA 表达呈负相关。该研究的 DS 持续时间短，排除了不成熟的 NT 形式，限制了全面的见解。GDNF 与 NT3 一起，可能在 DS 对情绪的反应中发挥重要作用。DS 期间的 PA 似乎会损害白细胞中 NTs 的 mRNA 表达。未来关于睡眠剥夺的研究可能会考虑在其明显冗余的情况下研究 BDNF 和 NT4 之间的关系。
