Department of Dermatology and Venereology, Västmanlands Sjukhus Västerås, 721 89 Västerås, Sweden.
Department of Dermatology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden.
Int J Mol Sci. 2024 Oct 3;25(19):10677. doi: 10.3390/ijms251910677.
Vitamin D plays an important role in psoriasis, but its involvement in pathogenesis remains unclear. This study aimed to evaluate vitamin D receptor (VDR) expression on peripheral blood mononuclear cells (PBMCs) in patients with psoriasis and healthy controls and to study the effects of the Etanercept treatment on VDR expression on PBMCs in patients with psoriasis. Twenty patients with moderate to severe psoriasis received treatment with Etanercept for 24 weeks. The age- and sex-matched controls did not receive any intervention. VDR expression on CD3 lymphocytes and CD14 monocytes, and serum levels of total and free 25-hydroxyvitamin D (25(OH)D) and 1,25-dihydroxy vitamin D (1,25(OH)D) were analyzed at baseline, after 10-12 weeks, and after 24 weeks in both groups. VDR expression was analyzed using flow cytometry. We observed higher expression of the VDR on CD14 monocytes in psoriasis patients compared to healthy controls at baseline. This difference was no longer significant after 24 weeks of the Etanercept treatment. The patients with psoriasis improved clinically. However, VDR expression was unaltered during the Etanercept treatment, and there was no correlation between VDR expression and disease severity.
维生素 D 在银屑病中起着重要作用，但它在发病机制中的作用仍不清楚。本研究旨在评估银屑病患者和健康对照者外周血单个核细胞（PBMC）中维生素 D 受体（VDR）的表达，并研究依那西普治疗对银屑病患者 PBMC 中 VDR 表达的影响。20 例中重度银屑病患者接受依那西普治疗 24 周。年龄和性别匹配的对照组未接受任何干预。在基线、治疗 10-12 周和 24 周时，分析两组患者 CD3 淋巴细胞和 CD14 单核细胞上的 VDR 表达以及总 25-羟维生素 D（25(OH)D）和 1,25-二羟维生素 D（1,25(OH)D）的血清水平。使用流式细胞术分析 VDR 表达。我们观察到银屑病患者 CD14 单核细胞上的 VDR 表达高于健康对照组，在依那西普治疗 24 周后，这种差异不再显著。银屑病患者的临床症状得到改善。然而，在依那西普治疗期间 VDR 表达没有改变，VDR 表达与疾病严重程度之间也没有相关性。
