Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, 518055 Shenzhen, China.
Chem Soc Rev. 2018 Feb 19;47(4):1391-1458. doi: 10.1039/c5cs00649j.
This critical review summarizes the developments in the integration of micro-optical elements with microfluidic platforms for facilitating detection and automation of bio-analytical applications. Micro-optical elements, made by a variety of microfabrication techniques, advantageously contribute to the performance of an analytical system, especially when the latter has microfluidic features. Indeed the easy integration of optical control and detection modules with microfluidic technology helps to bridge the gap between the macroscopic world and chip-based analysis, paving the way for automated and high-throughput applications. In our review, we start the discussion with an introduction of microfluidic systems and micro-optical components, as well as aspects of their integration. We continue with a detailed description of different microfluidic and micro-optics technologies and their applications, with an emphasis on the realization of optical waveguides and microlenses. The review continues with specific sections highlighting the advantages of integrated micro-optical components in microfluidic systems for tackling a variety of analytical problems, like cytometry, nucleic acid and protein detection, cell biology, and chemical analysis applications.
这篇评论综述了将微光学元件与微流控平台集成以促进生物分析应用的检测和自动化的发展。微光学元件由各种微纳加工技术制成，有利于提高分析系统的性能，尤其是当后者具有微流控特征时。实际上，光学控制和检测模块与微流控技术的轻松集成有助于弥合宏观世界与基于芯片的分析之间的差距，为自动化和高通量应用铺平了道路。在我们的综述中，我们首先介绍微流控系统和微光学组件，以及它们的集成方面。接下来，我们详细描述了不同的微流控和微光学技术及其应用，重点介绍了光波导和微透镜的实现。然后，我们继续有针对性地介绍集成微光学元件在微流控系统中的优势，以解决各种分析问题，如细胞分析、核酸和蛋白质检测、细胞生物学和化学分析应用。
