Eastern Region Branch, Taiwan Livestock Research Institute, Ministry of Agriculture, Taitung City, Taiwan.
Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Anim Biotechnol. 2024 Nov;35(1):2414299. doi: 10.1080/10495398.2024.2414299. Epub 2024 Oct 16.
The present study utilized full-length 16S rRNA gene sequencing to investigate the impact of dietary protein content on the composition and function of gut microbiota, and to analyze the gut microbiota of pigs in the growing (30 kg) and finishing (120 kg) stages under different feeding conditions. The results indicated that the gut microbiota was significantly different between pigs fed high- and low-protein diets. Comparing fecal samples from pigs at 30 and 120 kg, pigs at 30 kg showed a significant increase in the relative abundance of , whereas at 120 kg, the abundance of and decreased. To access the functional profiles and metabolic pathways based on amplicon sequence variants (ASVs), the microbiome of the 120 kg exhibited significant enrichments in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to metabolism-related category, including Alanine, aspartate and glutamate metabolism, Tyrosine and Thiamin metabolism, and Inositol phosphate metabolism. Meanwhile, analysis using the MetaCyc database showed that the metabolic pathways of the 30 kg group were significantly distinct when compared to the 120 kg of fecal samples. Overall, the findings indicated that the gut microbiota composition and function in the 30 and 120 kg fecal samples were markedly shaped by different dietary protein levels.
本研究利用全长 16S rRNA 基因测序技术，研究了饲粮蛋白水平对猪肠道微生物组成和功能的影响，并分析了不同饲养条件下生长（30kg）和育肥（120kg）阶段猪的肠道微生物。结果表明，饲粮蛋白水平对猪肠道微生物有显著影响。比较 30kg 和 120kg 猪的粪便样本，发现 30kg 猪的 相对丰度显著增加，而 120kg 猪的 和 丰度则下降。为了基于扩增子序列变异（ASV）获取功能图谱和代谢途径，120kg 猪的微生物组在与代谢相关类别相关的京都基因与基因组百科全书（KEGG）途径中显著富集，包括丙氨酸、天冬氨酸和谷氨酸代谢、酪氨酸和硫胺素代谢以及肌醇磷酸盐代谢。同时，使用 MetaCyc 数据库进行分析表明，与 120kg 粪便样本相比，30kg 组的代谢途径存在显著差异。总之，这些发现表明，30kg 和 120kg 粪便样本的肠道微生物组成和功能受不同饲粮蛋白水平的显著影响。
