含酪蛋白磷酸肽-无定形磷酸钙的氟化物清漆联合二极管激光照射对白斑病变再矿化的疗效：一项体外研究。

Efficacy of fluoride varnish containing casein phosphopeptide-amorphous calcium phosphate application and diode laser irradiation on white spot lesions remineralization: An in vitro study.

作者信息

El Sayed Mona, ElNaghy Rahma, Fathi Toka Hesham, Zeid Reem

机构信息

Conservative Dentistry Department, Faculty of Dentistry, Ahram Canadian University, Giza, Egypt.

Division of Graduate Orthodontics, University of Detroit Mercy School of Dentistry, 2700 Martin Luther King Jr. Blvd, Detroit, Michigan 48208, USA.

出版信息

Int Orthod. 2025 Mar;23(1):100929. doi: 10.1016/j.ortho.2024.100929. Epub 2024 Oct 15.

DOI:10.1016/j.ortho.2024.100929

PMID:39413554

Abstract

BACKGROUND/OBJECTIVES: The prevention of white spot lesions (WSLs) during orthodontic treatment with fixed appliances is of paramount importance to orthodontists. Numerous non-invasive techniques have been extensively researched to effectively manage WSLs. The objective of this study was to investigate the efficacy of MI varnish application, diode laser irradiation and their combination on remineralization of WSLs.

MATERIALS AND METHODS

In this in vitro study, 40 enamel samples were divided randomly into 4 groups based on the applied treatment after demineralization. In Group I, MI varnish was applied to the enamel samples for a period of 7 days. In Group II, samples were irradiated with diode laser. In Group III, MI varnish was applied for 7 days, followed by diode laser irradiation. Whereas Group IV (control group) received no treatment and samples were stored in artificial saliva for 7 days. Microhardness assessments were performed at baseline, after demineralization and following the different treatment protocols. A representative sample from each group was randomly selected for scanning electron microscopy (SEM) analysis. One-way ANOVA, repeated measures ANOVA, and Tukey's Post Hoc tests were used for statistical analysis, with significance set at P≤0.05.

RESULTS

All treatment groups demonstrated significantly higher mean microhardness values compared to the control group (P<0.05). Group 1 (MI varnish) had a final microhardness mean value of 193.50 (P<0.001), Group 2 (diode laser) recorded the highest final mean of 214.20 (P=0.018), and Group 3 (MI varnish+diode laser) had a mean of 203.93 (P=0.011). SEM observations supported these findings by showing enhanced surface morphology in the treatment groups.

CONCLUSIONS

MI varnish application, laser irradiation, and their combination demonstrated enhanced microhardness of artificially demineralized enamel, highlighting their effectiveness in remineralization of WSLs.

摘要

背景/目的：在固定矫治器正畸治疗期间预防白斑病变（WSLs）对正畸医生至关重要。众多非侵入性技术已被广泛研究以有效管理WSLs。本研究的目的是调查应用MI树脂、二极管激光照射及其联合应用对WSLs再矿化的疗效。

材料与方法

在这项体外研究中，40个釉质样本在脱矿后根据所应用的治疗方法随机分为4组。在第I组中，将MI树脂应用于釉质样本7天。在第II组中，样本接受二极管激光照射。在第III组中，应用MI树脂7天，随后进行二极管激光照射。而第IV组（对照组）不接受任何治疗，样本在人工唾液中保存7天。在基线、脱矿后以及不同治疗方案后进行显微硬度评估。从每组中随机选择一个代表性样本进行扫描电子显微镜（SEM）分析。采用单因素方差分析、重复测量方差分析和Tukey事后检验进行统计分析，显著性设定为P≤0.05。

结果

与对照组相比，所有治疗组的平均显微硬度值均显著更高（P<0.05）。第1组（MI树脂）的最终显微硬度平均值为193.50（P<0.001），第2组（二极管激光）记录的最终平均值最高，为214.20（P=0.018），第3组（MI树脂+二极管激光）的平均值为203.93（P=0.011）。SEM观察通过显示治疗组表面形态的改善支持了这些发现。