Wang Licheng, Chen Shaojin, Xing Mei, Dong Lingzhi, Zhu Huaxiong, Lin Yujin, Li Jinyi, Sun Tuo, Zhu Xiong, Wang Xiaoxia
Clinical & Central Laboratory of Sanya People's Hospital, Sanya, China.
Wenchang People's Hospital, Wenchang, China.
Front Microbiol. 2024 Oct 2;15:1474871. doi: 10.3389/fmicb.2024.1474871. eCollection 2024.
is an emerging marine zoonotic pathogen. In this study, we first reported the infections in patients and animals in Hainan Province, China. Currently, there is still relatively little known about the whole-genome characteristics of in most tropical regions, including in southern China. Here, we sequenced the 62 strains isolated from Hainan Province and combined with the whole genomes sequences of 144 genomes from public databases to analyze genomic features. Phylogenetic analysis revealed that is widely distributed in the marine environments of both temperate and tropical countries, exhibiting close phylogenetic relationships with genomes isolated from patients, animals, and plants. Thereby confirming that exposure to marine environments is a risk factor for infections. Average nucleotide identity analysis indicated that the clonally identical genomes could be isolated from patients with different sample types at different times. Pan-genome analysis identified a total of 21,909 genes, including 1,563 core genes, 8,292 strain-specific genes, and 12,054 accessory genes. Multiple putative virulence-associated genes were identified, encompassing 14 categories and 16 subcategories, with 171 distinct virulence factors. Three different plasmid replicon types were detected in 33 genomes. Eleven classes of antibiotic resistance genes and 352 integrons were identified. Antimicrobial susceptibility testing revealed a high resistance rate to imipenem and colistin among the strains studied, with 5 strains exhibiting multidrug resistance. However, they were all sensitive to amikacin, minocycline, and tigecycline. Our findings clarify the genomic characteristics and population structure of in Hainan Province. The results offer insights into the genetic basis of pathogenicity in and enhance our understanding of its global phylogeography.
是一种新兴的海洋人畜共患病原体。在本研究中，我们首次报道了在中国海南省患者和动物中的感染情况。目前，在包括中国南方在内的大多数热带地区，对于其全基因组特征仍知之甚少。在此，我们对从海南省分离出的62株菌株进行了测序，并结合来自公共数据库的144个基因组的全基因组序列来分析基因组特征。系统发育分析表明，在温带和热带国家的海洋环境中广泛分布，与从患者、动物和植物中分离出的基因组呈现出密切的系统发育关系。从而证实接触海洋环境是感染的一个风险因素。平均核苷酸同一性分析表明，可以在不同时间从不同样本类型的患者中分离出克隆相同的基因组。泛基因组分析共鉴定出21,909个基因，包括1,563个核心基因、8,292个菌株特异性基因和12,054个辅助基因。鉴定出多个假定的毒力相关基因，涵盖14个类别和16个子类别，有171种不同的毒力因子。在33个基因组中检测到三种不同的质粒复制子类型。鉴定出11类抗生素抗性基因和352个整合子。抗菌药物敏感性测试显示，在所研究的菌株中对亚胺培南和黏菌素的耐药率很高，有5株表现出多重耐药性。然而，它们对阿米卡星、米诺环素和替加环素均敏感。我们的研究结果阐明了海南省的基因组特征和种群结构。这些结果为致病性的遗传基础提供了见解，并增强了我们对其全球系统地理学的理解。
