Department of Basic Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, 10250, Sri Lanka.
Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, 10250, Sri Lanka.
J Appl Microbiol. 2024 Nov 4;135(11). doi: 10.1093/jambio/lxae262.
We have characterized the microbiome of infected chronic diabetic wounds (CDWs), exploring associations with antibiotic use and wound severity in a Sri Lankan cohort.
Fifty CDW patients were enrolled, 38 of whom received antibiotics. Tissue biopsies were analysed by microbiome profiling, and wounds were graded using the University of Texas Wound Grading System. Biofilm presence was assessed in 20 wounds. The microbiome was largely dominated by Enterobacteriaceae, Pseudomonadaceae, Streptococcaceae, and Corynebacteriaceae. Proteobacteria levels were significantly higher in antibiotic-treated wounds (P = .019), with increased Pseudomonas abundance. Wounds were categorized as grade 1 (10), grade 2 (29), and grade 3 (11). Alpha diversity varied by wound grade (P = .015), with grade 2 wounds showing the highest diversity and grade 3 the lowest. All 20 tested wounds were biofilm-positive, and community composition varied more in antibiotic-treated wounds (P = .004).
CDW microbiomes were dominated by Enterobacteriaceae and Pseudomonadaceae, with elevated Proteobacteria in antibiotic-treated wounds. Alpha diversity correlated with wound severity, peaking in grade 2 wounds. The high prevalence of biofilms in wounds underscores the need for management of CDWs that address microbial complexity.
我们对感染性慢性糖尿病性伤口（CDW）的微生物组进行了特征分析，在斯里兰卡队列中探索了其与抗生素使用和伤口严重程度的关联。
共纳入 50 例 CDW 患者，其中 38 例接受了抗生素治疗。通过微生物组谱分析对组织活检进行分析，并使用德克萨斯大学伤口分级系统对伤口进行分级。评估了 20 个伤口的生物膜存在情况。微生物组主要由肠杆菌科、假单胞菌科、链球菌科和棒状杆菌科主导。抗生素治疗的伤口中变形菌门水平显著升高（P=.019），假单胞菌丰度增加。伤口分为 1 级（10 例）、2 级（29 例）和 3 级（11 例）。伤口分级与 alpha 多样性有关（P=.015），2 级伤口的多样性最高，3 级伤口的多样性最低。所有 20 个测试的伤口均为生物膜阳性，且抗生素治疗的伤口中群落组成差异更大（P=.004）。
CDW 微生物组主要由肠杆菌科和假单胞菌科主导，抗生素治疗的伤口中变形菌门水平升高。alpha 多样性与伤口严重程度相关，在 2 级伤口中达到峰值。伤口中生物膜的高患病率强调了需要管理 CDW，以解决微生物复杂性。
