Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
BMC Vet Res. 2024 Oct 18;20(1):476. doi: 10.1186/s12917-024-04268-5.
Zinc oxide nanoparticles (ZnO NPs) have recently been applied in various veterinary and medical fields, however, the toxicological evaluations of these NPs in dogs are lacking. Therefore, the current study is designed to assess the impact of exposure to daily subcutaneous (SC) injections of ZnO NPs at different concentrations on various organs of mongrel dogs. Nine dogs were randomly divided into three groups (n = 3 for each) as follows: group (1) served as the control group, whereas groups (2&3) received SC injections of 50 and 100 ppm ZnO NPs (8 and 16 μg/kg bwt), respectively, once/day for 7 days. Our results revealed that ZnO NPs disrupted the oxidant/antioxidant balance in the lungs, liver, and kidneys of dogs in a dose-dependent manner. ZnO NPs induced dose-dependent radiological, ultrasonographical, and histopathological alterations in various organs especially lungs, spleen, liver, and kidneys along with disturbance in both liver and kidney biomarkers levels. Most organs of both ZnO NPs receiving groups displayed strong caspase-3 protein expression. Additionally, it upregulates the transcriptase levels of TNF-α and VEGF, as well as downregulates the antiapoptotic gene IL-10 in lung, kidney, and liver tissue homogenates. It was concluded that the daily SC injections of dogs with ZnO NPs at concentrations of 50 and 100 ppm caused extensive oxidative stress damage in various organs which provoked serious pathological processes such as apoptosis and inflammation.
氧化锌纳米粒子（ZnO NPs）最近已被应用于兽医和医学的多个领域，但这些 NPs 在犬类中的毒理学评估却很缺乏。因此，本研究旨在评估每天经皮下（SC）注射不同浓度 ZnO NPs 对杂种犬各器官的影响。9 只犬被随机分为三组（每组 3 只）：第 1 组作为对照组，第 2 组和第 3 组分别接受每天一次的 50 和 100 ppm ZnO NPs（8 和 16 μg/kg bwt）的 SC 注射，共 7 天。我们的结果表明，ZnO NPs 以剂量依赖的方式破坏了犬肺、肝和肾中的氧化应激/抗氧化平衡。ZnO NPs 诱导了各种器官的放射性、超声和组织病理学变化，特别是肺、脾、肝和肾，同时还扰乱了肝和肾生物标志物的水平。两个 ZnO NPs 组的大多数器官都显示出强烈的 caspase-3 蛋白表达。此外，它上调了 TNF-α 和 VEGF 的转录水平，并下调了肺、肾和肝组织匀浆中抗凋亡基因 IL-10 的水平。结论是，每天以 50 和 100 ppm 的浓度向犬皮下注射 ZnO NPs，会导致各种器官广泛的氧化应激损伤，引发严重的病理过程，如细胞凋亡和炎症。
