感染O:3血清型小肠结肠炎耶尔森菌的噬菌体vB_YenP_AP5的全基因组序列
Complete genome sequence of bacteriophage vB_YenP_AP5 which infects Yersinia enterocolitica of serotype O:3.
作者信息
Leon-Velarde Carlos G, Kropinski Andrew M, Chen Shu, Abbasifar Arash, Griffiths Mansel W, Odumeru Joseph A
机构信息
Laboratory Services Division, University of Guelph, Guelph, ON N1H 8J7, Canada.
出版信息
Virol J. 2014 Oct 28;11:188. doi: 10.1186/1743-422X-11-188.
BACKGROUND
Bacteriophage vB_YenP_AP5 is a lytic bacteriophage capable of infecting Yersinia enterocolitica strains of serotype O:3, an epidemiologically significant serotype within this bacterial species that causes yersiniosis in humans. This work describes the complete genome sequence of this phage.
RESULTS
The genome consists of linear double-stranded DNA of 38,646 bp, with direct terminal repeats of 235 bp in length, and a GC content of 50.7%. There are 45 open reading frames which occupy 89.9% of the genome. Most of the proteins encoded by this virus exhibit sequence similarity to Yersinia phage φYeO3-12 and Salmonella phage φSG-JL2 proteins.
CONCLUSIONS
Genomic and morphological analyses place the bacteriophage vB_YenP_AP5 in the T7likevirus genus of the subfamily Autographivirinae within the family Podoviridae.
背景
噬菌体vB_YenP_AP5是一种裂解性噬菌体，能够感染O:3血清型的小肠结肠炎耶尔森菌菌株，该血清型在这个细菌物种中具有重要的流行病学意义，可导致人类耶尔森菌病。这项工作描述了该噬菌体的完整基因组序列。
结果
基因组由38,646 bp的线性双链DNA组成，具有长度为235 bp的直接末端重复序列，GC含量为50.7%。有45个开放阅读框，占基因组的89.9%。该病毒编码的大多数蛋白质与耶尔森菌噬菌体φYeO3-12和沙门氏菌噬菌体φSG-JL2蛋白质具有序列相似性。
结论
基因组和形态学分析将噬菌体vB_YenP_AP5置于短尾病毒科自剪接病毒亚科的T7样病毒属中。
Zotero 插件